| IED ID | IndEnz0001000037 |
| Enzyme Type ID | amylase000037 |
| Protein Name |
Crossover junction endodeoxyribonuclease RuvC EC 3.1.21.10 Holliday junction nuclease RuvC Holliday junction resolvase RuvC |
| Gene Name | ruvC Cgl1662 cg1871 |
| Organism | Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Corynebacteriaceae Corynebacterium Corynebacterium glutamicum (Brevibacterium saccharolyticum) Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) |
| Enzyme Sequence | MNHEGLRVMGIDPGLTRCGLSVVQAGRGRTVYPVSVGVVRTPPDAELAERLLRLSKAVGEWMDEYTPDVIAIERVFERGNVSTVMNTAHAVGVLILAAAERGLPVHMYTPSEVKKAISGNGRADKKQMTVMITRILGLGEPPKPADAADALSLAVCHCWRAPMLMRAQSQYSEQELEKRRRVQQGKLGKAKSTYNAEQAQSHASDPAKAAHPSQFQRTDTN |
| Enzyme Length | 221 |
| Uniprot Accession Number | Q9AE11 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).; EC=3.1.21.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00034}; |
| DNA Binding | |
| EC Number | 3.1.21.10 |
| Enzyme Function | FUNCTION: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group. {ECO:0000255|HAMAP-Rule:MF_00034}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (1); Erroneous initiation (1); Metal binding (4); Region (1) |
| Keywords | DNA damage;DNA recombination;DNA repair;Hydrolase;Magnesium;Metal-binding;Nuclease;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 24,097 |
| Kinetics | |
| Metal Binding | METAL 12; /note=Magnesium; /evidence=ECO:0000255|HAMAP-Rule:MF_00034; METAL 73; /note=Magnesium; /evidence=ECO:0000255|HAMAP-Rule:MF_00034; METAL 146; /note=Magnesium; /evidence=ECO:0000255|HAMAP-Rule:MF_00034; METAL 149; /note=Magnesium; /evidence=ECO:0000255|HAMAP-Rule:MF_00034 |
| Rhea ID | |
| Cross Reference Brenda |