| IED ID | IndEnz0001000060 |
| Enzyme Type ID | amylase000060 |
| Protein Name |
Oligo-1,6-glucosidase IMA1 EC 3.2.1.10 Alpha-glucosidase Isomaltase 1 |
| Gene Name | IMA1 YGR287C |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MTISSAHPETEPKWWKEATFYQIYPASFKDSNDDGWGDMKGIASKLEYIKELGADAIWISPFYDSPQDDMGYDIANYEKVWPTYGTNEDCFALIEKTHKLGMKFITDLVINHCSSEHEWFKESRSSKTNPKRDWFFWRPPKGYDAEGKPIPPNNWKSYFGGSAWTFDEKTQEFYLRLFCSTQPDLNWENEDCRKAIYESAVGYWLDHGVDGFRIDVGSLYSKVVGLPDAPVVDKNSTWQSSDPYTLNGPRIHEFHQEMNQFIRNRVKDGREIMTVGEMQHASDETKRLYTSASRHELSELFNFSHTDVGTSPLFRYNLVPFELKDWKIALAELFRYINGTDCWSTIYLENHDQPRSITRFGDDSPKNRVISGKLLSVLLSALTGTLYVYQGQELGQINFKNWPVEKYEDVEIRNNYNAIKEEHGENSEEMKKFLEAIALISRDHARTPMQWSREEPNAGFSGPSAKPWFYLNDSFREGINVEDEIKDPNSVLNFWKEALKFRKAHKDITVYGYDFEFIDLDNKKLFSFTKKYNNKTLFAALNFSSDATDFKIPNDDSSFKLEFGNYPKKEVDASSRTLKPWEGRIYISE |
| Enzyme Length | 589 |
| Uniprot Accession Number | P53051 |
| Absorption | |
| Active Site | ACT_SITE 215; /note=Nucleophile; ACT_SITE 277; /note=Proton donor |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.; EC=3.2.1.10; |
| DNA Binding | |
| EC Number | 3.2.1.10 |
| Enzyme Function | FUNCTION: Major isomaltase (alpha-1,6-glucosidase) required for isomaltose utilization. Preferentially hydrolyzes isomaltose, palatinose, and methyl-alpha-glucoside, with little activity towards isomaltotriose or longer oligosaccharides. Does not hydrolyze maltose. {ECO:0000269|PubMed:15291818, ECO:0000269|PubMed:20562106}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (23); Chain (1); Helix (29); Initiator methionine (1); Mutagenesis (4); Sequence conflict (1); Site (1); Turn (7) |
| Keywords | 3D-structure;Direct protein sequencing;Glycosidase;Hydrolase;Maltose metabolism;Mitochondrion;Reference proteome |
| Interact With | P39940 |
| Induction | INDUCTION: Expression is increased in response to the addition of maltose, isomaltose, and alpha-methylglucopyranoside. {ECO:0000269|PubMed:20562106}. |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14576278}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (5) |
| Cross Reference PDB | 3A47; 3A4A; 3AJ7; 3AXH; 3AXI; |
| Mapped Pubmed ID | 10466139; 16606443; 16702403; 16823961; 17665192; 20443596; 20471265; 20518737; 20886742; 21216897; 21535483; 21925939; 21998701; 22416705; 22448915; 23239941; 24453942; 24465730; 24649402; |
| Motif | |
| Gene Encoded By | |
| Mass | 68,592 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.10; |