Detail Information for IndEnz0001000061
IED ID IndEnz0001000061
Enzyme Type ID amylase000061
Protein Name Oligo-1,6-glucosidase IMA4
EC 3.2.1.10
Alpha-glucosidase
Flocculent-specific protein 2
Isomaltase 4
Gene Name IMA4 FSP2 YJL221C HRE589 J0218
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Enzyme Sequence MTISSAHPETEPKWWKEATIYQIYPASFKDSNNDGWGDMKGIASKLEYIKELGTDAIWISPFYDSPQDDMGYDIANYEKVWPTYGTNEDCFALIEKTHKLGMKFITDLVINHCSSEHEWFKESRSSKTNPKRDWFFWRPPKGYDAEGKPIPPNNWRSYFGGSAWTFDEKTQEFYLRLFCSTQPDLNWENEDCRKAIYESAVGYWLDHGVDGFRIDVGSLYSKVAGLPDAPVIDENSKWQLSDPFTMNGPRIHEFHQEMNKFIRNRVKDGREIMTVGEMRHATDETKRLYTSASRHELSELFNFSHTDVGTSPKFRQNLIPYELKDWKVALAELFRYVNGTDCWSTIYLENHDQPRSITRFGDDSPKNRVISGKLLSVLLVSLSGTLYVYQGQELGEINFKNWPIEKYEDVEVRNNYDAIKEEHGENSKEMKRFLEAIALISRDHARTPMQWSREEPNAGFSGPNAKPWFYLNESFREGINAEDESKDPNSVLNFWKEALRFRKAHKDITVYGYDFEFIDLDNKKLFSFTKKYDNKTLFAALNFSSDSIDFTIPNNSSSFKLEFGNYPRSEVDASSRTLKPWEGRIYISE
Enzyme Length 589
Uniprot Accession Number P0CW41
Absorption
Active Site ACT_SITE 215; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 277; /note=Proton donor; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.; EC=3.2.1.10;
DNA Binding
EC Number 3.2.1.10
Enzyme Function FUNCTION: Alpha-glucosidase with broad substrate specificity for alpha-1,4- and alpha-1,6-glucosides. Not required for isomaltose utilization, but overexpression allows the IMA1 null mutant to grow on isomaltose. {ECO:0000269|PubMed:20562106}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Site (1)
Keywords Glycosidase;Hydrolase;Maltose metabolism;Reference proteome
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 12702356; 18983662; 20443596; 20471265; 20518737; 20886742; 22416705; 23239941; 24453942;
Motif
Gene Encoded By
Mass 68,699
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda