Detail Information for IndEnz0001000099
IED ID IndEnz0001000099
Enzyme Type ID amylase000099
Protein Name Cupincin
EC 3.4.-.-
52 kDa globulin-like protein
allergen Ory s NRA
Gene Name Os03g0793700 LOC_Os03g57960 OSJNBb0060J21.10
Organism Oryza sativa subsp. japonica (Rice)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice)
Enzyme Sequence MAKKKTSSSMARSQLAALLISLCFLSLASNAVGWSRRGEREEEDERRRHGGEGGRPYHLGEESFRHWTRTRHGRFSVLERFPDEQVVGAAVGGYRVAVLEAAPRAFLQPSHYDADEVFYVKEGEGVIVLLREGRRESFCVREGDAMVIPAGAIVYSANTHSSKWFRVVMLLNPVSTPGHFEEYFPVGGDRPESFFSAFSDDVLQAAFNTRREELEKVFERQREGGEITTAPEEQIRELSKSCSRGGGGGSGSEWEIKPSSLTGKSPYFSNNHGKLFELTGDECRHLKKLDLQIGLANITRGSMIAPNYNTRATKLAVVLQGSGYFEMACPHVSGGGSSERREREREHGRRREEEQGEEEHGERGEKARRYHKVRAQVREESVIVIPASHPATIVASEGESLAVVCFFVGANHDEKVFLAGRNSPLRQLDDPAKKLVFGGSAAREADRVLAAQPEQILLRGPHGRGSVSDM
Enzyme Length 470
Uniprot Accession Number Q852L2
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.-.-
Enzyme Function FUNCTION: Seed storage protein (Probable). Globulin-like protein that acts as zinc metalloprotease. Cleaves specifically between Leu-15 and Tyr-16 of insulin B chain, and Gln-1 and Leu-2 of neurotensin (NT) peptide in vitro. May play a role as an initiating endopeptidase in germinating seeds (By similarity). {ECO:0000250|UniProtKB:B8AL97, ECO:0000305}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Compositional bias (1); Domain (2); Glycosylation (1); Metal binding (3); Region (3); Sequence conflict (4); Signal peptide (1)
Keywords Allergen;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Seed storage protein;Signal;Storage protein;Zinc
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..34; /evidence=ECO:0000250|UniProtKB:B8AL97
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 52,126
Kinetics
Metal Binding METAL 347; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:B8AL97; METAL 352; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:B8AL97; METAL 360; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:B8AL97
Rhea ID
Cross Reference Brenda