| IED ID | IndEnz0001000115 |
| Enzyme Type ID | amylase000115 |
| Protein Name |
Probable alpha-amylase 2 AtAMY2 EC 3.2.1.1 1,4-alpha-D-glucan glucanohydrolase |
| Gene Name | AMY2 At1g76130 T23E18.6 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MGYYNNVFDECNDQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK |
| Enzyme Length | 413 |
| Uniprot Accession Number | Q8LFG1 |
| Absorption | |
| Active Site | ACT_SITE 193; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P04063; ACT_SITE 218; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P04063 |
| Activity Regulation | |
| Binding Site | BINDING 220; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 222; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P04063; BINDING 239; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 246; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 280; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 299; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 305; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 386; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 411; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.; EC=3.2.1.1; Evidence={ECO:0000250|UniProtKB:P00693}; |
| DNA Binding | |
| EC Number | 3.2.1.1 |
| Enzyme Function | FUNCTION: Probable alpha-amylase that does not seem to be required for breakdown of transitory starch in leaves. {ECO:0000269|PubMed:15637061}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (9); Chain (1); Erroneous gene model prediction (1); Region (3); Site (1) |
| Keywords | Carbohydrate metabolism;Cytoplasm;Glycosidase;Hydrolase;Metal-binding;Reference proteome |
| Interact With | |
| Induction | INDUCTION: Circadian-regulated, with a peak in expression at the beginning of the light period. {ECO:0000269|PubMed:15347792}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14996213; 15665241; 15749471; 15862090; 17324226; 19250611; 23393426; 23909862; |
| Motif | |
| Gene Encoded By | |
| Mass | 47,170 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |