| IED ID | IndEnz0001000169 |
| Enzyme Type ID | amylase000169 |
| Protein Name |
Glycinin G1 Glycinin 11S G1 Glycinin A1aB1b allergen Gly m 6 Cleaved into: Glycinin A1a subunit Glycinin acidic 1a subunit ; Glycinin Bx subunit Glycinin basic x subunit Glycinin B1b subunit Glycinin basic 1b subunit |
| Gene Name | GY1 Glyma03g32030 |
| Organism | Glycine max (Soybean) (Glycine hispida) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Fabales Fabaceae Papilionoideae 50 kb inversion clade NPAAA clade indigoferoid/millettioid clade Phaseoleae Glycine Glycine subgen. Soja Glycine max (Soybean) (Glycine hispida) |
| Enzyme Sequence | MAKLVFSLCFLLFSGCCFAFSSREQPQQNECQIQKLNALKPDNRIESEGGLIETWNPNNKPFQCAGVALSRCTLNRNALRRPSYTNGPQEIYIQQGKGIFGMIYPGCPSTFEEPQQPQQRGQSSRPQDRHQKIYNFREGDLIAVPTGVAWWMYNNEDTPVVAVSIIDTNSLENQLDQMPRRFYLAGNQEQEFLKYQQEQGGHQSQKGKHQQEEENEGGSILSGFTLEFLEHAFSVDKQIAKNLQGENEGEDKGAIVTVKGGLSVIKPPTDEQQQRPQEEEEEEEDEKPQCKGKDKHCQRPRGSQSKSRRNGIDETICTMRLRHNIGQTSSPDIYNPQAGSVTTATSLDFPALSWLRLSAEFGSLRKNAMFVPHYNLNANSIIYALNGRALIQVVNCNGERVFDGELQEGRVLIVPQNFVVAARSQSDNFEYVSFKTNDTPMIGTLAGANSLLNALPEEVIQHTFNLKSQQARQIKNNNPFKFLVPPQESQKRAVA |
| Enzyme Length | 495 |
| Uniprot Accession Number | P04776 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Glycinin is the major seed storage protein of soybean (PubMed:2485233). Glycinin basic peptides (GBPs), and, to a lower extent, glycinin exhibit antibacterial activity against Gram-negative and Gram-positive bacteria (e.g. L.monocytogenes, B.subtilis, E.coli and S.enteritidis) by forming pores and aggregating in transmembranes, leading to membrane permeability and, eventually, cell death (PubMed:22236762, Ref.15, PubMed:28590128). {ECO:0000269|PubMed:22236762, ECO:0000269|PubMed:2485233, ECO:0000269|PubMed:28590128, ECO:0000269|Ref.15}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (2); Beta strand (26); Chain (2); Compositional bias (1); Disulfide bond (2); Domain (2); Erroneous gene model prediction (1); Helix (9); Motif (1); Propeptide (2); Region (2); Sequence conflict (6); Signal peptide (1); Turn (3) |
| Keywords | 3D-structure;Allergen;Alternative splicing;Disulfide bond;Endoplasmic reticulum;Reference proteome;Seed storage protein;Signal;Storage protein;Vacuole |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000269|PubMed:29348620}. Protein storage vacuole {ECO:0000269|PubMed:29348620}. Note=Hexamers are assembled in the endoplasmic reticulum and later sorted to the protein storage vacuoles. {ECO:0000269|PubMed:29348620}. |
| Modified Residue | |
| Post Translational Modification | PTM: During soybean germination, seed storage proteins are hydrolyzed by protease/26S proteasome. {ECO:0000269|PubMed:29037738}.; PTM: The precursor is post-translationally processed to form a covalently linked A1a-Bx subunit complex. |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (3) |
| Cross Reference PDB | 1FXZ; 1UCX; 1UD1; |
| Mapped Pubmed ID | 21959908; |
| Motif | MOTIF 486..495; /note=Vacuolar targeting signal; /evidence=ECO:0000269|PubMed:29348620 |
| Gene Encoded By | |
| Mass | 55,706 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |