| IED ID | IndEnz0001000178 |
| Enzyme Type ID | amylase000178 |
| Protein Name |
Beta-amylase 5 AtBeta-Amy EC 3.2.1.2 1,4-alpha-D-glucan maltohydrolase Protein REDUCED BETA AMYLASE 1 |
| Gene Name | BAM5 BMY1 RAM1 At4g15210 dl3650c FCAALL.97 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MATNYNEKLLLNYVPVYVMLPLGVVNVENVFADPETLETQLKRLKEEAGVDGVMVDVWWGIIESKGPKQYDWTAYKTLFQLIARLGLKIQAIMSFHQCGGNVGDIVTIPIPQWVRDVGDNDPDIYYTNRKGTRDIEYLSIGVDNLPLFAGRTAVQLYSDYMSSFKENMADLIEAGVIVDIEVGLGPAGELRYPSYPQSQGWVFPGIGEFQCYDKYLKKDFKEAAAKAGHPEWDLPEDAGEYNDKPEETGFFKKDGTYVSEKGKFFMTWYSNKLIFHGDQILGEANKIFAGLKVNLAAKVSGIHWLYNHHSHAAELTAGYYNLFKRDGYRPIARMLSKHYGILNFTCLEMKDTDNTAEALSAPQELVQEVLSKAWKEGIEVAGENALETYGAKGYNQILLNARPNGVNPNGKPKLRMYGFTYLRLSDTVFQENNFELFKKLVRKMHADQDYCGDAAKYGHEIVPLKTSNSQLTLEDIADAAQPSGAFKWDSETDLKVDG |
| Enzyme Length | 498 |
| Uniprot Accession Number | P25853 |
| Absorption | |
| Active Site | ACT_SITE 189; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10050; ACT_SITE 383; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10050 |
| Activity Regulation | |
| Binding Site | BINDING 56; /note=Substrate; /evidence=ECO:0000250; BINDING 96; /note=Substrate; /evidence=ECO:0000250; BINDING 104; /note=Substrate; /evidence=ECO:0000250; BINDING 298; /note=Substrate; /evidence=ECO:0000250; BINDING 303; /note=Substrate; /evidence=ECO:0000250; BINDING 345; /note=Substrate; /evidence=ECO:0000250; BINDING 423; /note=Substrate; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.; EC=3.2.1.2; |
| DNA Binding | |
| EC Number | 3.2.1.2 |
| Enzyme Function | FUNCTION: Beta-amylase activity. Major cytosolic beta-amylase isoform in rosette leaves and inflorescences stems. {ECO:0000269|PubMed:11743123}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (2); Binding site (7); Chain (1); Region (1); Sequence conflict (4) |
| Keywords | Alternative splicing;Carbohydrate metabolism;Cytoplasm;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome |
| Interact With | |
| Induction | INDUCTION: Circadian-regulated, with a peak in expression just before the light period in short day conditions. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:8552713}. Note=Present in the continuous phloem cytoplasm. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10652124; 10982426; 11292072; 12047632; 12805584; 15347792; 15665241; 15749471; 15753106; 15862090; 15960623; 16297066; 18650403; 19251652; 20118269; 20153546; 21637781; 23393426; 24561248; 25293962; 28627464; 29066669; 30348315; 32072133; 32254060; 8434930; 9161039; 9193090; |
| Motif | |
| Gene Encoded By | |
| Mass | 56,063 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |