Detail Information for IndEnz0001000227
IED ID IndEnz0001000227
Enzyme Type ID amylase000227
Protein Name 1,4-alpha-glucan branching enzyme GlgB
EC 2.4.1.18
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-
1-
4
-glucan branching enzyme
Glycogen branching enzyme
BE
Gene Name glgB Bxeno_B0164 Bxe_B2863
Organism Paraburkholderia xenovorans (strain LB400)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Betaproteobacteria Burkholderiales Burkholderiaceae Paraburkholderia Paraburkholderia xenovorans Paraburkholderia xenovorans (strain LB400)
Enzyme Sequence MSEHDPAAGLQPLDIDALVEARHPDPFSQLGLHHTDAGPVVRALLPNAAHVSVISRADGALLGELEQLRPGLFAGRITSAAPYRLRIDWHGVVQEIEDTYSFGPVLGDEPLGRLAGGDPYAVLECLGARPMEVDGVPGVRFAVWAPNARRVSVVGDFNAWDGRRHPMRLRHQAGVWELFVPRVGPGTRYKYELLSRDGHPLPLKADPCAMQTEKPPGTASIVAHVDEVEQFPWSDHEWIQSRAGKQTARSPISIYEVHAESWLRVAEEGQRGLDWEELAERMIPYVKSMGFTHVEFMPIAEHPFGGSWGYQPLGQFAPSARFGKPEQFARFVDKAHEAGLGVILDWVPAHFPNDAHGLIDFDGTPLYEHADPREGYHQDWNTMIYNLGRNEVSAFLIASGLAWLKRYHVDGLRVDAVASMLYRDYSRAADQWVPNIYGGRENLESIAFLKRLNHEVGYVPGVPGAITIAEESTAWPGVTARVEDGGLGFQFKWNMGWMHDTLHYMEEDPVYRQYHHHNMTFGMVYAYSERFVLPLSHDEVVHGKGSLLGKMPGDRWQKFANLRAYFGFMWTHPGKKLLFMGGEFGQLAEFNHDASPHWHLLDDSNHHGVQMLVRDLNRLYSDEPALYLLDCEPGGFEWLIGDDSGNSVFAYRRTDGAGRELVVVCNMTPVPRLGYRIGMPRGGRWVEVLNTDAGVYGGSNMGNGGLIHTDSQSSHGWPHSAALTLPPLATIVLRAD
Enzyme Length 736
Uniprot Accession Number Q13S07
Absorption
Active Site ACT_SITE 415; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_00685; ACT_SITE 470; /note=Proton donor; /evidence=ECO:0000255|HAMAP-Rule:MF_00685
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.; EC=2.4.1.18; Evidence={ECO:0000255|HAMAP-Rule:MF_00685};
DNA Binding
EC Number 2.4.1.18
Enzyme Function FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. {ECO:0000255|HAMAP-Rule:MF_00685}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan biosynthesis; glycogen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00685}.
nucleotide Binding
Features Active site (2); Chain (1)
Keywords Carbohydrate metabolism;Glycogen biosynthesis;Glycogen metabolism;Glycosyltransferase;Reference proteome;Transferase
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 82,135
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda