IED ID | IndEnz0001000242 |
Enzyme Type ID | amylase000242 |
Protein Name |
Alpha-amylase 2 EC 3.2.1.1 1,4-alpha-D-glucan glucanohydrolase 2 |
Gene Name | SWA2 |
Organism | Schwanniomyces occidentalis (Yeast) (Debaryomyces occidentalis) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Debaryomycetaceae Schwanniomyces Schwanniomyces occidentalis (Yeast) (Debaryomyces occidentalis) |
Enzyme Sequence | MKFATILSTTALALSSLVASKPIFLSKRDAGSSAAAAWRSESIYQLVTDRFARTDGSTSATCNTGDRVYCGGTFQGIIDKLDYIQGMGFTAIWISPVVEQIPDDTGYGYAYHGYWMKDIYAINSNFGTADDLKNLSNELHKRNMKLMVDIVTNHYAWNGAGSSVAYSNYNPFNQQSYFHDYCLITNYDDQTNVEDCWEGDNTVSLPDLRTEDSDVSSIFNLWVAELVSNYSIDGLRIDSAKHVDESFYPSFQSAAGVYLLGEVYDGDPAYTCPYQNYMSGVTNYPLYYPMLRFFQGTSNSVDELNAMISSLESDCKDITLLGNFIENHDQPRLPSYTSDSALIKNAIAFNLMSDGIPIIYYGQEQGYSGSSDPNNREALWLSGYSTSNGYYKLISSVNQIRNQAIYKDSKYTTYWSDVLYASGHVIALQRGADDQRIVSVFNNLGSSGSQTVTFSTKYSGGEKVVDVLTCQTSYANSDSTLTVSISGGAPRIYAPASLIANSGICNF |
Enzyme Length | 507 |
Uniprot Accession Number | Q08806 |
Absorption | |
Active Site | ACT_SITE 238; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P56271; ACT_SITE 262; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P56271 |
Activity Regulation | |
Binding Site | BINDING 115; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P0C1B3; BINDING 154; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P0C1B3; BINDING 236; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P0C1B3; BINDING 266; /note=Substrate; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:P0C1B3; BINDING 329; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P0C1B3; BINDING 376; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P0C1B3 |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.; EC=3.2.1.1; |
DNA Binding | |
EC Number | 3.2.1.1 |
Enzyme Function | |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Binding site (6); Chain (1); Disulfide bond (4); Glycosylation (1); Metal binding (6); Region (1); Signal peptide (1); Site (1) |
Keywords | Calcium;Carbohydrate metabolism;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Metal-binding;Signal |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 55,967 |
Kinetics | |
Metal Binding | METAL 153; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:P0C1B3; METAL 194; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P56271; METAL 207; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:P56271; METAL 238; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:P0C1B3; METAL 242; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P0C1B3; METAL 262; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:P0C1B3 |
Rhea ID | |
Cross Reference Brenda |