| IED ID | IndEnz0001000244 |
| Enzyme Type ID | amylase000244 |
| Protein Name |
Alpha-amylase isozyme 3A EC 3.2.1.1 1,4-alpha-D-glucan glucanohydrolase |
| Gene Name | AMY1.2 AMY3A Os09g0457400 LOC_Os09g28400 B1045B05.8 OsJ_29629 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MGKQMAALCGFLLVALLWLTPDVAHAQTQILFQGFNWDSWKKQGGWYNMLKDQVGDIASAGVTHVWLPPPTHSVSPQGYMPGRLYDLNASKYGTKAELKSLIAAFHAKGIKCVADIVVNHRCADDKDGRGVYCIFKGGGPRGCLDWGPSMICCDDTQYSDGTGHRDTGADFAAAPDIDHLNPLVQRELSDWLRWLRRDVGFDGWRLDFAKGYSAAVARTYVQNARPSFVVAEIWNSLSYDGDGKPAANQDGQRQELVNWVKQVGGPATAFDFTTKGILQSAVQGELWRMRDKDGKAPGMIGWYPEKAVTFVDNHDTGSTQRMWPFPSDKVILGYAYILTHPGVPCIFYDHVFDWNLKQEINALAATRKRNGINAGSKLRVLAAESDMYVAMVDERVITKIGPRIDVGNIIPSDFHIVAHGNDYCVWEKSGLRVPEPEGRR |
| Enzyme Length | 440 |
| Uniprot Accession Number | P27932 |
| Absorption | |
| Active Site | ACT_SITE 207; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P00693; ACT_SITE 232; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P00693 |
| Activity Regulation | |
| Binding Site | BINDING 234; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 236; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P04063; BINDING 254; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 295; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 314; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 320; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 399; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 426; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.; EC=3.2.1.1; Evidence={ECO:0000250|UniProtKB:P00693}; |
| DNA Binding | |
| EC Number | 3.2.1.1 |
| Enzyme Function | FUNCTION: Important for breakdown of endosperm starch during germination. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (8); Chain (1); Metal binding (11); Region (6); Sequence conflict (2); Signal peptide (1); Site (1) |
| Keywords | Calcium;Carbohydrate metabolism;Glycosidase;Hydrolase;Metal-binding;Reference proteome;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 22967050; 27438877; |
| Motif | |
| Gene Encoded By | |
| Mass | 48,868 |
| Kinetics | |
| Metal Binding | METAL 119; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:P00693; METAL 145; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:P00693; METAL 155; /note=Calcium 3; /evidence=ECO:0000250|UniProtKB:P00693; METAL 166; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:P00693; METAL 169; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P00693; METAL 170; /note=Calcium 3; /evidence=ECO:0000250|UniProtKB:P00693; METAL 171; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P00693; METAL 174; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P00693; METAL 176; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:P00693; METAL 176; /note=Calcium 3; /evidence=ECO:0000250|UniProtKB:P00693; METAL 211; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P00693 |
| Rhea ID | |
| Cross Reference Brenda |