| IED ID | IndEnz0001000245 |
| Enzyme Type ID | amylase000245 |
| Protein Name |
Alpha-amylase isozyme 3C EC 3.2.1.1 1,4-alpha-D-glucan glucanohydrolase |
| Gene Name | AMY1.7 AMY3B Os09g0457800 LOC_Os09g28420 LOC_Os09g28430 B1045B05.11 OsJ_29632 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MAKHSTTMSCLLFFVLLCLGSHLAQAQVLFQGFNWESWKKQGGWYNFLHSHVDDIAATGVTHVWLPPPSHSVAPQGYMPGRLYDLDASKYGTGAELRSLIAAFHSKSIKCVADIVINHRCADYKDSRGIYCIFEGGTPDSRLDWGPDMICSDDTQYSNGRGHRDTGADFGAAPDIDHLNTRVQTELSDWLNWLKSDVGFDGWRLDFAKGYSATVAKTYVDNTDPSFVVAEIWSNMRYDGNGEPSWNQDGDRQELVNWAQAVGGPASAFDFTTKGELQAAVQGELWRMKDGNGKAPGMIGWLPEKAVTFIDNHDTGSTQNSWPFPSDKVMQGYAYILTHPGVPCIFYDHVFDWNLKQEISTLAAVRSRNGIHPGSKLNILAADGDVYVAMIDDKVITKIGTRYDVGNLIPSDFHVVAHGNNYCVWEKSGLRVPAGRRH |
| Enzyme Length | 437 |
| Uniprot Accession Number | P27939 |
| Absorption | |
| Active Site | ACT_SITE 205; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P00693; ACT_SITE 230; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P00693 |
| Activity Regulation | |
| Binding Site | BINDING 232; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 234; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 252; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 293; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 312; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 318; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 397; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 424; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.; EC=3.2.1.1; Evidence={ECO:0000250|UniProtKB:P00693}; |
| DNA Binding | |
| EC Number | 3.2.1.1 |
| Enzyme Function | FUNCTION: Important for breakdown of endosperm starch during germination. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (8); Chain (1); Metal binding (14); Region (6); Sequence conflict (4); Signal peptide (1); Site (1) |
| Keywords | Calcium;Carbohydrate metabolism;Glycosidase;Hydrolase;Metal-binding;Reference proteome;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 23845039; |
| Motif | |
| Gene Encoded By | |
| Mass | 48,504 |
| Kinetics | |
| Metal Binding | METAL 117; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:P00693; METAL 134; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:P00693; METAL 137; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P00693; METAL 139; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P00693; METAL 143; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:P00693; METAL 153; /note=Calcium 3; /evidence=ECO:0000250|UniProtKB:P00693; METAL 164; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:P00693; METAL 167; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P00693; METAL 168; /note=Calcium 3; /evidence=ECO:0000250|UniProtKB:P00693; METAL 169; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P00693; METAL 172; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P00693; METAL 174; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:P00693; METAL 174; /note=Calcium 3; /evidence=ECO:0000250|UniProtKB:P00693; METAL 209; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P00693 |
| Rhea ID | |
| Cross Reference Brenda |