| IED ID | IndEnz0001000248 |
| Enzyme Type ID | amylase000248 |
| Protein Name |
Alpha-amylase type B isozyme EC 3.2.1.1 1,4-alpha-D-glucan glucanohydrolase Clone 103 Fragment |
| Gene Name | AMY1.4 |
| Organism | Hordeum vulgare (Barley) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Pooideae Triticodae Triticeae Hordeinae Hordeum Hordeum vulgare (Barley) |
| Enzyme Sequence | ILNVAVEGALWRLRGTDGKAPSMIGWWPAKAVTFVDNHDTGSTQHMWPFPSDRVMQGYAYILTHPRTPCIFYDHFFDWGPKEEIDRLVSVRTRHGIHNESKLQIIEADADLYLAEIDGKVIVKLGPRYDVGNLIPGGFEGAAHGNDYAVWEKI |
| Enzyme Length | 153 |
| Uniprot Accession Number | P04748 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 19; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 38; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 44; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 123; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 150; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.; EC=3.2.1.1; Evidence={ECO:0000250|UniProtKB:P00693}; |
| DNA Binding | |
| EC Number | 3.2.1.1 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Binding site (5); Chain (1); Non-terminal residue (1); Region (1); Site (1) |
| Keywords | Calcium;Carbohydrate metabolism;Germination;Glycosidase;Hydrolase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 17,276 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |