| IED ID | IndEnz0001000302 |
| Enzyme Type ID | amylase000302 |
| Protein Name |
1,4-alpha-glucan branching enzyme GlgB EC 2.4.1.18 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase Alpha- 1- 4 -glucan branching enzyme Glycogen branching enzyme BE |
| Gene Name | glgB PFL_2874 |
| Organism | Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas fluorescens group (fluorescent pseudomonads) Pseudomonas protegens Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) |
| Enzyme Sequence | MSFSNKEQGKQQPALLPSAKAIDALVRAEHRDPFAILGPHGDGAGGQFIRAFLPGALSVQVLDRDQQQVLGSLQAGEVPGLFVGHFPEARPYLLRIQWAGGEQVSEDPYSFGPLLGEMDLYLFAEGNHRDLSSCLGAQLTTVDGIDGVRFAVWAPNARRVSVVGDFNIWDGRRHPMRLRHPSGVWELFIPRLQAGEAYKYEILGPQGILPLKADPMALATQLPPDTASRVAPPLTIDWQDQEWMLARGERQRVDAPLSIYELHAGSWQCEVDDLGEVARQYSWAELAERLIPYVKDLGFTHIELMPIMEHPFGGSWGYQPLSQFAPSARYGSPQDFAAFIDACHQAGIGVILDWVPAHFPTDTHGLAQFDGTALYEYGNPLEGFHQDWDTLIYNLGRTEVHGFMLASALHWLKHFHVDGLRVDAVASMLYRDYSRKAGEWVPNRHGGRENLEAIDFLRHLNDVVALEAPGALVIAEESTAWPGVSQRTDQGGLGFAYKWNMGWMHDSLHYIQQDPVYRAHHHNELSFGLVYAWSERFILPISHDEVVHGKHSLIDKMPGDRWQKFANLRAYLSFMWAHPGKKLLFMGCEFGQWREWNHDQQLDWYLLQYPEHRGVQQLVGDLNRLYRQYPPLHDQDDVPQGFQWLIGDDAINSVYAWLRWSKSGEPLLVVANFTPVPRQGYRVGVPVAGRWSELLNSDSQLYAGSNYGNGGEAFSEEQSSHGQALSLVLNLPPLAVLILRPDS |
| Enzyme Length | 743 |
| Uniprot Accession Number | Q4KCQ3 |
| Absorption | |
| Active Site | ACT_SITE 423; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_00685; ACT_SITE 476; /note=Proton donor; /evidence=ECO:0000255|HAMAP-Rule:MF_00685 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.; EC=2.4.1.18; Evidence={ECO:0000255|HAMAP-Rule:MF_00685}; |
| DNA Binding | |
| EC Number | 2.4.1.18 |
| Enzyme Function | FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. {ECO:0000255|HAMAP-Rule:MF_00685}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan biosynthesis; glycogen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00685}. |
| nucleotide Binding | |
| Features | Active site (2); Chain (1) |
| Keywords | Carbohydrate metabolism;Glycogen biosynthesis;Glycogen metabolism;Glycosyltransferase;Reference proteome;Transferase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 83,646 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |