IED ID | IndEnz0001000309 |
Enzyme Type ID | amylase000309 |
Protein Name |
Trypsin inhibitor ClTI EC 3.4.21.4 Fragment |
Gene Name | |
Organism | Cassia leiandra (Marimari) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Fabales Fabaceae Caesalpinioideae Cassia clade Cassia Cassia leiandra (Marimari) |
Enzyme Sequence | SVELDSDGEPIRNGGGLYYILPVVQGKGGGLEFAKTGSQS |
Enzyme Length | 40 |
Uniprot Accession Number | C0HK48 |
Absorption | |
Active Site | |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.; EC=3.4.21.4; Evidence={ECO:0000269|Ref.1}; |
DNA Binding | |
EC Number | 3.4.21.4 |
Enzyme Function | FUNCTION: Inhibits trypsin but not chymotrypsin, papain or porcine pancreatic alpha-amylase. Has insecticidal activity against A.aegypti. Functions by inhibiting the A.aegypti midgut proteases to reduce the survival of larva and adults. {ECO:0000269|Ref.1}. |
Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Stable at temperatures up to 70 degrees Celsius. Incubation at temperatures above 70 degrees Celsius steadily decreases inhibitory activity. {ECO:0000269|Ref.1}; |
PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Stable in the pH range 2.2-10.0. {ECO:0000269|Ref.1}; |
Pathway | |
nucleotide Binding | |
Features | Chain (1); Non-terminal residue (1) |
Keywords | Direct protein sequencing;Hydrolase;Protease inhibitor;Serine protease inhibitor |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 4,097 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |