Detail Information for IndEnz0001000312
IED ID IndEnz0001000312
Enzyme Type ID amylase000312
Protein Name Oligo-1,6-glucosidase
EC 3.2.1.10
Dextrin 6-alpha-D-glucanohydrolase
Oligosaccharide alpha-1,6-glucosidase
Sucrase-isomaltase
Isomaltase
Gene Name malL
Organism Bacillus cereus
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus cereus group Bacillus cereus
Enzyme Sequence MEKQWWKESVVYQIYPRSFMDSNGDGIGDLRGIISKLDYLKELGIDVIWLSPVYESPNDDNGYDISDYCKIMNEFGTMEDWDELLHEMHERNMKLMMDLVVNHTSDEHNWFIESRKSKDNKYRDYYIWRPGKEGKEPNNWGAAFSGSAWQYDEMTDEYYLHLFSKKQPDLNWDNEKVRQDVYEMMKFWLEKGIDGFRMDVINFISKEEGLPTVETEEEGYVSGHKHFMNGPNIHKYLHEMNEEVLSHYDIMTVGEMPGVTTEEAKLYTGEERKELQMVFQFEHMDLDSGEGGKWDVKPCSLLTLKENLTKWQKALEHTGWNSLYWNNHDQPRVVSRFGNDGMYRIESAKMLATVLHMMKGTPYIYQGEEIGMTNVRFESIDEYRDIETLNMYKEKVMERGEDIEKVMQSIYIKGRDNARTPMQWDDQNHAGFTTGEPWITVNPNYKEINVKQAIQNKDSIFYYYKKLIELRKNNEIVVYGSYDLILENNPSIFAYVRTYGVEKLLVIANFTAEECIFELPEDISYSEVELLIHNYDVENGPIENITLRPYEAMVFKLK
Enzyme Length 558
Uniprot Accession Number P21332
Absorption
Active Site ACT_SITE 199; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 255; /note=Proton donor; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.; EC=3.2.1.10;
DNA Binding
EC Number 3.2.1.10
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Beta strand (21); Chain (1); Helix (28); Metal binding (4); Site (1); Turn (4)
Keywords 3D-structure;Calcium;Cytoplasm;Direct protein sequencing;Glycosidase;Hydrolase;Metal-binding
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (1)
Cross Reference PDB 1UOK;
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 66,013
Kinetics
Metal Binding METAL 21; /note=Calcium; /evidence=ECO:0000250|UniProtKB:O06994; METAL 23; /note=Calcium; /evidence=ECO:0000250|UniProtKB:O06994; METAL 25; /note=Calcium; /evidence=ECO:0000250|UniProtKB:O06994; METAL 29; /note=Calcium; /evidence=ECO:0000250|UniProtKB:O06994
Rhea ID
Cross Reference Brenda