| IED ID | IndEnz0001000317 |
| Enzyme Type ID | amylase000317 |
| Protein Name |
Pullulanase 1, chloroplastic AtPU1 EC 3.2.1.142 Protein LIMIT DEXTRINASE AtLDA |
| Gene Name | PU1 LDA At5g04360 T19N18.90 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MALTLTPTSSVHLLSSISVARPRIFAADFNLRSRWRRRRPVTSISNFRLRLPSKTSLHCLCSSSSASSPMSLEVSSPNSQFLDCLIYSRAYWVTQGVIAWNVDVGEGSCYFYASKSAGLSFSEDGIDGYDLRIKLEAESGSLPADVIEKFPHIRNYKSFKVPKDLDIRDLVKSQLAVVCFDAEGRLIQGTGLQLPGVLDELFSYDGPLGAHFTPEGVSLHLWAPTAQAVSVCIYKNPLDKSPMEICPLKEANGVWSTEGACSWGGCYYVYKVSVYHPSTMKLETCYANDPYARGLSADGRKTFLVNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPENRGGYLAFTSKDSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQARITEIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKMVQALNCTGLNVVLDVVYNHLHASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVNYKVDGFRFDLMGHIMKATIVNAKSAIGSLRKETDGVDGSRIYLYGEGWNFGEVAENGRGINASQFNLGGTGIGSFNDRIRDATLGGSPFGHPLQQGFITGLLLQPNAHDHGSEATQELMLSTAKNHIQTGMAANLKDYMLTNHEGKEVKGSEVLMHDATPVAYASLPTETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLASSMIALSQGIPFFHAGDEILRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKGKNEHNWPLIKPRLQDPSFKPKSSHIVATLHNFLDLLRIRYSSPLFRLDTARAIQERVRFHNTGPSSIPGAIVMSIEDGHRGIPSVSQIDPIYSLIVVIFNARPSEFSYPSPALKDRKLELHPVQVMSADEIVKKSVYDSFSGGFTVPARTTTVFVESRNG |
| Enzyme Length | 965 |
| Uniprot Accession Number | Q8GTR4 |
| Absorption | |
| Active Site | ACT_SITE 552; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 589; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in alpha- and beta-limit dextrins of amylopectin and glycogen, and in amylopectin and pullulan.; EC=3.2.1.142; |
| DNA Binding | |
| EC Number | 3.2.1.142 |
| Enzyme Function | FUNCTION: Involved in starch degradation and also probably in the trimming of pre-amylopectin chains during starch synthesis. {ECO:0000269|PubMed:16495218, ECO:0000269|PubMed:18815382, ECO:0000269|PubMed:19074683}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan biosynthesis; starch biosynthesis.; PATHWAY: Glycan degradation; starch degradation. |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Sequence conflict (1); Site (1); Transit peptide (1) |
| Keywords | Carbohydrate metabolism;Chloroplast;Glycosidase;Hydrolase;Plastid;Reference proteome;Starch biosynthesis;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000269|PubMed:16495218, ECO:0000269|PubMed:18431481}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14576160; 15028209; 15347792; 15749471; 15862090; 15927942; 16045475; 17028209; 18650403; 19776162; 22789914; 22916167; 23019330; 23393426; 26896394; 28152100; 28627464; |
| Motif | |
| Gene Encoded By | |
| Mass | 107,067 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |