Detail Information for IndEnz0001000321
IED ID IndEnz0001000321
Enzyme Type ID amylase000321
Protein Name 4-alpha-glucanotransferase
EC 2.4.1.25
Amylomaltase
Disproportionating enzyme
D-enzyme
Gene Name mgtA TM_0364
Organism Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
Taxonomic Lineage cellular organisms Bacteria Thermotogae Thermotogae Thermotogales Thermotogaceae Thermotoga Thermotoga maritima Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
Enzyme Sequence MIGYQIYVRSFRDGNLDGVGDFRGLKNAVSYLKELGIDFVWLMPVFSSISFHGYDVVDFYSFKAEYGSEREFKEMIEAFHDSGIKVVLDLPIHHTGFLHTWFQKALKGDPHYRDYYVWANKETDLDERREWDGEKIWHPLEDGRFYRGLFGPFSPDLNYDNPQVFDEMKRLVLHLLDMGVDGFRFDAAKHMRDTIEQNVRFWKYFLSDLKGIFLAEIWAEARMVDEHGRIFGYMLNFDTSHCIKEAVWKENTRVLIESIERAVIGKDYLPVNFTSNHDMSRLASFEGGFSKEKIKLSISILFTLPGVPLVFYGDELGMKGVYQKPNTEVVLDPFPWNESMCVEGQTFWKWPAYNGPFSGISVEYQKRDPDSILSHTLGWTRFRKENQWIDRAKLEFLCKEDKFLVYRLYDDQHSLKVFHNLSGEEVVFEGVKMKPYKTEVV
Enzyme Length 441
Uniprot Accession Number P80099
Absorption
Active Site ACT_SITE 186; /note=Nucleophile; ACT_SITE 216; /note=Proton donor
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.; EC=2.4.1.25;
DNA Binding
EC Number 2.4.1.25
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Beta strand (25); Chain (1); Helix (18); Metal binding (5); Sequence conflict (1); Site (1); Turn (5)
Keywords 3D-structure;Calcium;Carbohydrate metabolism;Cytoplasm;Direct protein sequencing;Glycosyltransferase;Metal-binding;Reference proteome;Transferase
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (2)
Cross Reference PDB 1LWH; 1LWJ;
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 51,843
Kinetics
Metal Binding METAL 13; /note=Calcium; METAL 15; /note=Calcium; METAL 17; /note=Calcium; METAL 19; /note=Calcium; via carbonyl oxygen; METAL 21; /note=Calcium
Rhea ID
Cross Reference Brenda 2.4.1.25;