Detail Information for IndEnz0001000322
IED ID IndEnz0001000322
Enzyme Type ID amylase000322
Protein Name 4-alpha-glucanotransferase
EC 2.4.1.25
Amylomaltase
Disproportionating enzyme
D-enzyme
Maltodextrin glycosyltransferase
Gene Name mgtA
Organism Thermotoga neapolitana
Taxonomic Lineage cellular organisms Bacteria Thermotogae Thermotogae Thermotogales Thermotogaceae Thermotoga Thermotoga neapolitana
Enzyme Sequence MIGYQIYVRSFRDGNFDGVGDFKGLKGAISYLKELGVDFVWLMPVFSSISFHGYDVVDFYSFKAEYGDEKDFREMIEAFHDNGIKVVLDLPIHHTGFLHTWFQKALKGDPHYRDYYVWASEKTDLDERREWDNERIWHPLEDGRFYRGLFGPLSPDLNYDNPQVFEEMKKVVYHLLEMGVDGFRFDAAKHMRDTLEQNVRFWRYFLSDIEGIFLAEIWAESKVVDEHGRIFGYMLNFDTSHCIKEAVWKENFKVLIESIERALVGKDYLPVNFTSNHDMSRLASFEGGLSEEKVKLSLSILFTLPGVPLIFYGDELGMKGIYRKPNTEVVLDPFPWSENISLEGQTFWKWPAYNSPFSGVSVEYQKKKRDSILLHIMKWTGFRKENHWLDRANIEFLCKEEKLLHVYRLVDEGRSLKVIHNLSNGEMVFEGVRVQPYSTEVI
Enzyme Length 442
Uniprot Accession Number O86956
Absorption
Active Site ACT_SITE 186; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 216; /note=Proton donor; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.; EC=2.4.1.25; Evidence={ECO:0000269|Ref.1};
DNA Binding
EC Number 2.4.1.25
Enzyme Function FUNCTION: Hydrolyzes the 1,4-alpha-glycoside bonds in oligomeric and polymeric 1,4-alpha-glucans and transfers oligosaccharides (maltotriose being the shortest one) to acceptor maltodextrins. {ECO:0000269|Ref.1}.
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 85 degrees Celsius. {ECO:0000269|Ref.1};
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.0. {ECO:0000269|Ref.1};
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (5); Site (1)
Keywords Calcium;Carbohydrate metabolism;Cytoplasm;Glycosyltransferase;Metal-binding;Transferase
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 51,935
Kinetics
Metal Binding METAL 13; /note=Calcium; /evidence=ECO:0000250; METAL 15; /note=Calcium; /evidence=ECO:0000250; METAL 17; /note=Calcium; /evidence=ECO:0000250; METAL 19; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250; METAL 21; /note=Calcium; /evidence=ECO:0000250
Rhea ID
Cross Reference Brenda