Detail Information for IndEnz0001000325
IED ID IndEnz0001000325
Enzyme Type ID amylase000325
Protein Name Beta-amylase 8
EC 3.2.1.2
1,4-alpha-D-glucan maltohydrolase
Beta-amylase 2
Gene Name BAM8 BMY2 At5g45300 K9E15.8
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MHTLNNTITTTTGSQDPNLDPIPDPDQFPNRNRNQPQSRRPRGFAAAAAAASIAPTENDVNNGNIAGIGGGEGSSGGGGGGGGKGKREREKEKERTKLRERHRRAITSRMLAGLRQYGNFPLPARADMNDVIAALAREAGWSVEADGTTYRQSQQPNHVVQFPTRSIESPLSSSTLKNCAKAAIESQQHSVLRNDEKLAPVSLDSIGIAESDHPGNGRYTSVSPITSVGCLEANQLIQDVHSAEQCNDFTESFYVPVYAMLPVGIIDNFGQLVDPEGVRQELSYMKSLNVDGVVIDCWWGIVEGWNPQKYVWSGYRELFNLIRDFKLKLQVVMAFHEYGGNASGNVMISLPQWVLKIGKDNPDIFFTDREGRRSFECLNWSIDKERVLHGRTGIEVYFDFMRSFRSEFDDLFVEGLITAVEIGLGASGELKYPSFPERMGWIYPGIGEFQCYDKYSQLSLQKEAKSRGFTFWGKGPENAGQYSSHPHETVFFQERGEYDSYYGRFFLNWYSQLLIGHAENVLSLANLAFEETKIIVKIPAIYWSYKTASHAAELTAGYYNPSNRDGYSLVFETLKKYSVTVKFVCPGPQMSPNAHEEALADPEGLSWQVINAAWDKGLQIGGENAITCFDRDGCMRLIDIAKPRNHPDGYHFSFFTYRQPSPLVQGSTCFPDLDYFIKRMHGDIRDKQF
Enzyme Length 689
Uniprot Accession Number Q9FH80
Absorption
Active Site ACT_SITE 429; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 623; /note=Proton acceptor; /evidence=ECO:0000250
Activity Regulation
Binding Site BINDING 296; /note=Substrate; /evidence=ECO:0000250; BINDING 336; /note=Substrate; /evidence=ECO:0000250; BINDING 537; /note=Substrate; /evidence=ECO:0000250
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.; EC=3.2.1.2;
DNA Binding
EC Number 3.2.1.2
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Alternative sequence (1); Binding site (3); Chain (1); Compositional bias (2); Region (2)
Keywords Alternative splicing;Carbohydrate metabolism;Cytoplasm;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome
Interact With O80831
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 14666423; 14996213; 15287975; 15347792; 15749471; 16297066; 16698902; 16776300; 16798941; 18218973; 21487098; 21798944; 23393426; 24748042;
Motif
Gene Encoded By
Mass 77,160
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda