| IED ID | IndEnz0001000333 |
| Enzyme Type ID | amylase000333 |
| Protein Name |
Rqc2 homolog RqcH RqcH |
| Gene Name | rqcH yloA BSU15640 |
| Organism | Bacillus subtilis (strain 168) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168) |
| Enzyme Sequence | MSFDGMFTYGMTHELNEKIMGGRITKIHQPYKHDVIFHIRAKGKNQKLLLSAHPSYSRVHITAQAYENPSEPPMFCMLLRKHIEGGFIEKIEQAGLDRIMIFHIKSRNEIGDETVRKLYVEIMGRHSNIILTDAAENVIIDGLKHLSPSMNSYRTVLPGQDYKLPPAQDKISPLEASEDDILRHLSFQEGRLDKQIVDHFSGVSPLFAKEAVHRAGLANKVTLPKALLALFAEVKEHRFIPNITTVNGKEYFYLLELTHLKGEARRFDSLSELLDRFYFGKAERDRVKQQAQDLERFVVNERKKNANKIKKLEKTLEYSENAKEFQLYGELLTANLYMLKKGDKQAEVINYYDEESPTITIPLNPNKTPSENAQAYFTKYQKAKNSVAVVEEQIRLAQEEIEYFDQLIQQLSSASPRDISEIREELVEGKYLRPKQQKGQKKQKPHNPVLETYESTSGLTILVGKNNRQNEYLTTRVAARDDIWLHTKDIPGSHVVIRSSEPDEQTIMEAATIAAYFSKAKDSSSVPVDYTKIRHVKKPNGAKPGFVTYDSQHTVFVTPDADTVIKLKKS |
| Enzyme Length | 570 |
| Uniprot Accession Number | O34693 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Part of the ribosome quality control system (RQC). Recruits Ala-charged tRNA and directs the elongation of stalled nascent chains on 50S ribosomal subunits, leading to non-templated C-terminal Ala extensions (Ala tail). The Ala tail promotes nascent chain degradation. Selectively binds tRNA(Ala)(UGC), which is presumably the sole source of tRNA(Ala) used for Ala tailing directed by this protein. May add between 1 and at least 8 Ala residues; detection of the Ala tail requires either deletion of clpP or its inhibition. Binds to 50S ribosomal subunits, at least 30% of which contain a P-site tRNA and thus are obstructed. {ECO:0000269|PubMed:31155236}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (28); Chain (1); Erroneous initiation (3); Helix (18); Mutagenesis (3); Turn (3) |
| Keywords | 3D-structure;Protein biosynthesis;RNA-binding;Reference proteome;tRNA-binding |
| Interact With | |
| Induction | INDUCTION: Up-regulated under secretion stress conditions, possibly in a CssR/CssS-dependent manner. {ECO:0000269|PubMed:17586671}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | Electron microscopy (5) |
| Cross Reference PDB | 7AQC; 7AQD; 7AS8; 7AS9; 7ASA; |
| Mapped Pubmed ID | 33259810; 33259811; |
| Motif | |
| Gene Encoded By | |
| Mass | 65,205 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |