| IED ID | IndEnz0001000339 |
| Enzyme Type ID | amylase000339 |
| Protein Name |
Malto-oligosyltrehalose trehalohydrolase MTHase EC 3.2.1.141 4-alpha-D- 1- 4 -alpha-D-glucano trehalose trehalohydrolase Maltooligosyl trehalose trehalohydrolase |
| Gene Name | treZ SSO2093 |
| Organism | Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) |
| Taxonomic Lineage | cellular organisms Archaea TACK group Crenarchaeota Thermoprotei Sulfolobales Sulfolobaceae Saccharolobus Saccharolobus solfataricus (Sulfolobus solfataricus) Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) |
| Enzyme Sequence | MTFGYKLDEDGVTFNLWAPYQRKVKLKILNRGIYEMERDDKGYFTITLDNVRVGDRYKYILDDNSEVPDPASRYQPEGVHGYSEIISPDFEWDDENSVKVKREDLVIYELHIGTFTSEGTFEGVIKKLNYLKELGVTAIEIMPIAQFPGKKDWGYDGVYLYAVQNSYGGPSGFRKLVNEAHKLGLAVILDVVYNHVGPEGNYMVKLGPYFSEKYKTPWGLTFNFDDAGSDEVRKFILENVEYWINEFHVDGFRLDAVHAIIDNSPKHILEDIADVVHKYDKIVIAESDLNDPRVVNPKEKCGYNIDAQWVDDFHHAIHAFLTGERQGYYSDFGSIGDIVKSYKDVFIYDGKYSNFRRKTHGKSVGDLDGCKFVVYIQNHDQVGNRGGGERLIKLVDKESYKIAAALYILSPYIPMIFMGEEYGEENPFYYFSDFSDPKLIQGVREGRRRENGQETDPQSDCTFNDSKLSWKINDDILSFYKSLIKIRKEYGLACNRKLSVENGNYWLTVKGNGCLAVYVFSKSVIEMKYSGTLVLSSNSSFPSQITESKYELDKGFALYKL |
| Enzyme Length | 561 |
| Uniprot Accession Number | P95867 |
| Absorption | |
| Active Site | ACT_SITE 255; /note=Nucleophile; /evidence=ECO:0000305|PubMed:18563901; ACT_SITE 286; /note=Proton donor; /evidence=ECO:0000305|PubMed:18563901 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan.; EC=3.2.1.141; Evidence={ECO:0000269|PubMed:18563901}; |
| DNA Binding | |
| EC Number | 3.2.1.141 |
| Enzyme Function | |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 85 degrees Celsius. {ECO:0000269|PubMed:18563901}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.0. {ECO:0000269|PubMed:18563901}; |
| Pathway | PATHWAY: Glycan biosynthesis; trehalose biosynthesis. {ECO:0000305|PubMed:18563901}. |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Mutagenesis (8); Region (3); Site (1) |
| Keywords | Carbohydrate metabolism;Cytoplasm;Glycosidase;Hydrolase;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 64,371 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=7.22 mM for maltotriosyltrehalose {ECO:0000269|PubMed:18563901}; KM=5.66 mM for maltotetraosyltrehalose {ECO:0000269|PubMed:18563901}; KM=5.89 mM for maltopentaosyltrehalose {ECO:0000269|PubMed:18563901}; KM=5.86 mM for maltopentaose {ECO:0000269|PubMed:18563901}; KM=5.63 mM for maltohexaose {ECO:0000269|PubMed:18563901}; KM=2.63 mM for maltoheptaose {ECO:0000269|PubMed:18563901}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.141; |