Detail Information for IndEnz0001000339
IED ID IndEnz0001000339
Enzyme Type ID amylase000339
Protein Name Malto-oligosyltrehalose trehalohydrolase
MTHase
EC 3.2.1.141
4-alpha-D-
1-
4
-alpha-D-glucano
trehalose trehalohydrolase
Maltooligosyl trehalose trehalohydrolase
Gene Name treZ SSO2093
Organism Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus)
Taxonomic Lineage cellular organisms Archaea TACK group Crenarchaeota Thermoprotei Sulfolobales Sulfolobaceae Saccharolobus Saccharolobus solfataricus (Sulfolobus solfataricus) Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus)
Enzyme Sequence MTFGYKLDEDGVTFNLWAPYQRKVKLKILNRGIYEMERDDKGYFTITLDNVRVGDRYKYILDDNSEVPDPASRYQPEGVHGYSEIISPDFEWDDENSVKVKREDLVIYELHIGTFTSEGTFEGVIKKLNYLKELGVTAIEIMPIAQFPGKKDWGYDGVYLYAVQNSYGGPSGFRKLVNEAHKLGLAVILDVVYNHVGPEGNYMVKLGPYFSEKYKTPWGLTFNFDDAGSDEVRKFILENVEYWINEFHVDGFRLDAVHAIIDNSPKHILEDIADVVHKYDKIVIAESDLNDPRVVNPKEKCGYNIDAQWVDDFHHAIHAFLTGERQGYYSDFGSIGDIVKSYKDVFIYDGKYSNFRRKTHGKSVGDLDGCKFVVYIQNHDQVGNRGGGERLIKLVDKESYKIAAALYILSPYIPMIFMGEEYGEENPFYYFSDFSDPKLIQGVREGRRRENGQETDPQSDCTFNDSKLSWKINDDILSFYKSLIKIRKEYGLACNRKLSVENGNYWLTVKGNGCLAVYVFSKSVIEMKYSGTLVLSSNSSFPSQITESKYELDKGFALYKL
Enzyme Length 561
Uniprot Accession Number P95867
Absorption
Active Site ACT_SITE 255; /note=Nucleophile; /evidence=ECO:0000305|PubMed:18563901; ACT_SITE 286; /note=Proton donor; /evidence=ECO:0000305|PubMed:18563901
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan.; EC=3.2.1.141; Evidence={ECO:0000269|PubMed:18563901};
DNA Binding
EC Number 3.2.1.141
Enzyme Function
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 85 degrees Celsius. {ECO:0000269|PubMed:18563901};
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.0. {ECO:0000269|PubMed:18563901};
Pathway PATHWAY: Glycan biosynthesis; trehalose biosynthesis. {ECO:0000305|PubMed:18563901}.
nucleotide Binding
Features Active site (2); Chain (1); Mutagenesis (8); Region (3); Site (1)
Keywords Carbohydrate metabolism;Cytoplasm;Glycosidase;Hydrolase;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 64,371
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=7.22 mM for maltotriosyltrehalose {ECO:0000269|PubMed:18563901}; KM=5.66 mM for maltotetraosyltrehalose {ECO:0000269|PubMed:18563901}; KM=5.89 mM for maltopentaosyltrehalose {ECO:0000269|PubMed:18563901}; KM=5.86 mM for maltopentaose {ECO:0000269|PubMed:18563901}; KM=5.63 mM for maltohexaose {ECO:0000269|PubMed:18563901}; KM=2.63 mM for maltoheptaose {ECO:0000269|PubMed:18563901};
Metal Binding
Rhea ID
Cross Reference Brenda 3.2.1.141;