| IED ID | IndEnz0001000344 |
| Enzyme Type ID | amylase000344 |
| Protein Name |
Sucrose phosphorylase EC 2.4.1.7 Glucosyltransferase-A GTF-A Sucrose glucosyltransferase |
| Gene Name | gtfA SMU_881 |
| Organism | Streptococcus mutans serotype c (strain ATCC 700610 / UA159) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus Streptococcus mutans Streptococcus mutans serotype c (strain ATCC 700610 / UA159) |
| Enzyme Sequence | MPIINKTMLITYADSLGKNLKELNENIENYFGDAVGGVHLLPFFPSTGDRGFAPIDYHEVDSAFGDWDDVKCLGEKYYLMFDFMINHISRQSKYYKDYQEKHEASAYKDLFLNWDKFWPKNRPTQEDVDLIYKRKDRAPKQEIQFADGSVEHLWNTFGEEQIDLDVTKEVTMDFIRSTIENLAANGCDLIRLDAFAYAVKKLDTNDFFVEPEIWTLLDKVRDIAAVSGAEILPEIHEHYTIQFKIADHDYYVYDFALPMVTLYSLYSSKVDRLAKWLKMSPMKQFTTLDTHDGIGVVDVKDILTDEEITYTSNELYKVGANVNRKYSTAEYNNLDIYQINSTYYSALGDDDQKYFLARLIQAFAPGIPQVYYVGFLAGKNDLELLESTKEGRNINRHYYSSEEIAKEVKRPVVKALLNLFTYRNQSAAFDLDGRIEVETPNEATIVIERQNKDGSHIAKAEINLQDMTYRVTENDQTISFE |
| Enzyme Length | 481 |
| Uniprot Accession Number | P10249 |
| Absorption | |
| Active Site | ACT_SITE 193; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:A0ZZH6; ACT_SITE 234; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:A0ZZH6 |
| Activity Regulation | |
| Binding Site | BINDING 49; /note=Sucrose; /evidence=ECO:0000250|UniProtKB:A0ZZH6; BINDING 87; /note=Sucrose; /evidence=ECO:0000250|UniProtKB:A0ZZH6; BINDING 234; /note=Sucrose; /evidence=ECO:0000250|UniProtKB:A0ZZH6; BINDING 392; /note=Sucrose; /evidence=ECO:0000250|UniProtKB:A0ZZH6 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=phosphate + sucrose = alpha-D-glucose 1-phosphate + D-fructose; Xref=Rhea:RHEA:24048, ChEBI:CHEBI:17992, ChEBI:CHEBI:37721, ChEBI:CHEBI:43474, ChEBI:CHEBI:58601; EC=2.4.1.7; Evidence={ECO:0000269|PubMed:2971020};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:24049; Evidence={ECO:0000269|PubMed:2971020};PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:24050; Evidence={ECO:0000269|PubMed:2971020}; |
| DNA Binding | |
| EC Number | 2.4.1.7 |
| Enzyme Function | FUNCTION: Intracellular catabolism of sucrose (PubMed:2971020). Being intracellular, probably not involved in synthesis of extracellular polysaccharides (Probable). {ECO:0000269|PubMed:2971020, ECO:0000305|PubMed:2967248}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Initiator methionine (1); Mutagenesis (1); Region (3); Sequence conflict (15) |
| Keywords | Cytoplasm;Dental caries;Direct protein sequencing;Glycosyltransferase;Reference proteome;Transferase |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:2971020}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 55,687 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:24048; RHEA:24049; RHEA:24050 |
| Cross Reference Brenda |