| IED ID | IndEnz0001000357 |
| Enzyme Type ID | amylase000357 |
| Protein Name |
Isoamylase EC 3.2.1.68 |
| Gene Name | iam |
| Organism | Flavobacterium sp. |
| Taxonomic Lineage | cellular organisms Bacteria FCB group Bacteroidetes/Chlorobi group Bacteroidetes Flavobacteriia Flavobacteriales Flavobacteriaceae Flavobacterium unclassified Flavobacterium Flavobacterium sp. |
| Enzyme Sequence | MDPHAPQRQRSGQRLRALALAALACALSPAHAAIDAQQLGARYDAAQANLAFRVYSSRATRVEVFLYKNPTGSQEVARLALSKDPATQVWSLSLPTSTIKNTYGITGAVYYGYRAWGPNWPYDAAWTKGSATGFVSDVDNAGNRFNPNKLLLDPYAREISQDPNTATCADGTIYATGAAHRNKDSGLCASKGIALAADATSVGSKPTRALKDEVIYEVHVRGLTRNDDSVPAAERGTYKGAARKAAALAALGVTAVEFLPVQETQNDQNDVDPNSTAGDNYWGYMTLNYFAPDRRYAYDKSAGGPTREWKAMVKAFHDAGIKVYIDVVYNHTGEGGPWSGTDGLSVYNLLSFRGLDNPAYYSLSSDYKYPWDNTGVGGNYNTRHPIAQNLIVDSLAYWRDALGVDGFRFDLASVLGNSCQHGCFNFDKNDSGNALNRIVAELPPRPAAGGAGADLIAEPWAIGGNSYQVGGFPAGWAEWNGLYRDALRKKQNKLGVETVTPGTLATRFAGSNDLYGDDGRKPWHSINFVVAHDGFTLNDLYAYNDKQNNQPWPYGPSDGGEDHNLSWNQGGIVAEQRKAARTGLALLMLSAGVPMITGGDEALRTQFGNNNTYNLDSAANWLYWSRSALEADHETYTKRLIAFRKAHPALRPANFYSASDTNGNVMEQLRWFKPDGAQADSAYFNGADNHALAWRIDGSEFGDSASAIYVAYNGWSGAVDFKLPWPGTGKQWYRVTDTATWNEGPNAVALPGSETLIGGENTVYGMQARSLLLLIAK |
| Enzyme Length | 777 |
| Uniprot Accession Number | O32611 |
| Absorption | |
| Active Site | ACT_SITE 410; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 458; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen, amylopectin and their beta-limit dextrins.; EC=3.2.1.68; |
| DNA Binding | |
| EC Number | 3.2.1.68 |
| Enzyme Function | FUNCTION: Has a high rate of hydrolysis for glycogen. Does not cleave pullulan. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6-7.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (1); Metal binding (5); Signal peptide (1); Site (1) |
| Keywords | Calcium;Disulfide bond;Glycosidase;Hydrolase;Metal-binding;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..32; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 84,340 |
| Kinetics | |
| Metal Binding | METAL 162; /note=Calcium; /evidence=ECO:0000250; METAL 263; /note=Calcium; /evidence=ECO:0000250; METAL 264; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250; METAL 266; /note=Calcium; /evidence=ECO:0000250; METAL 293; /note=Calcium; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |