| IED ID | IndEnz0001000376 |
| Enzyme Type ID | amylase000376 |
| Protein Name |
Myricetin 3-O-rhamnosyltransferase UGT77B2 EC 2.4.1.- UDP-glucosyltransferase 1 CcUGT1 |
| Gene Name | UGT77B2 UGT1 |
| Organism | Crocosmia x crocosmiiflora (Montbretia) (Crocosmia aurea x Crocosmia pottsii) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae Asparagales Iridaceae Crocoideae Freesieae Crocosmia Crocosmia x crocosmiiflora (Montbretia) (Crocosmia aurea x Crocosmia pottsii) |
| Enzyme Sequence | MDSASRQHVALIAFPFASHPGNLFAFARALAAAAPDITFSFLTTTFAAATLPPAPPAANLRLCHVADGVPEGGLPPGTTIHGRIGMFLRATPGNFRDGVRAAEEEVGVKVSCVVSDAFLWMTADVAEEIGAQWLPLWTCAPAALLAHVSTDQLRERFGVEKQATAGWADELVDFIPGLSCLRIRDIPDEIVTNWHSDLSILLHRMGNQLTSATAVALNTFDGLDTTIDAALASLFKKTLPIGPLNLLSSPPPLQPGDEKCLSWLDGQEDATVAYVSFGTMVLMPTQSDVSEIAQGLESSGVRFLWSLREEARAGLLPPGFLERTAGRGLVVPWAPQVRVLGHRAVGAFVTHCGWNAVMESVTSGVPMACLPSFADQKTNARMVSAAWGIGEALRGEKVTKEEVVRSMEIVMMGEEGRRMRERIGNLREKAAEAVGPGGSSSENFKSVLEMVRGTAN |
| Enzyme Length | 456 |
| Uniprot Accession Number | A0A2Z5CVA1 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 19; /note=UDP-D-glucose; /evidence=ECO:0000250|UniProtKB:Q7XT97; BINDING 279; /note=UDP-D-glucose; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:Q7XT97 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=myricetin + UDP-beta-L-rhamnose = H(+) + myricetin 3-O-alpha-L-rhamnoside + UDP; Xref=Rhea:RHEA:61144, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:58395, ChEBI:CHEBI:83836, ChEBI:CHEBI:144432; Evidence={ECO:0000269|PubMed:29967287, ECO:0000269|PubMed:31004005};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:61145; Evidence={ECO:0000269|PubMed:29967287, ECO:0000269|PubMed:31004005}; |
| DNA Binding | |
| EC Number | 2.4.1.- |
| Enzyme Function | FUNCTION: Rhamnosyltransferase involved in montbretin A (MbA) biosynthesis (PubMed:29967287, PubMed:31004005). Catalyzes the 3-O rhamnosylation of myricetin to produce myricetin 3-O-alpha-L-rhamnoside (MR), a precursor of MbA (PubMed:29967287, PubMed:31004005). MbA is a potent inhibitor of human pancreatic alpha-amylase and is being developed as drug candidate to treat type-2 diabetes (PubMed:29967287, PubMed:31004005). In vitro, is able to transfer UDP-glucose and UDP-xylose with 50-fold less efficiency compared with UDP-rhamnose (PubMed:29967287). In vitro, can use kaempferol or quercetin as substrates, although these two flavonols may not be physiological substrates in vivo (PubMed:29967287). {ECO:0000269|PubMed:29967287, ECO:0000269|PubMed:31004005}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Flavonoid metabolism. {ECO:0000305}. |
| nucleotide Binding | |
| Features | Binding site (2); Chain (1); Region (2) |
| Keywords | Glycosyltransferase;Transferase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 48,737 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:61144; RHEA:61145 |
| Cross Reference Brenda |