| IED ID | IndEnz0001000379 |
| Enzyme Type ID | amylase000379 |
| Protein Name |
Malto-oligosyltrehalose trehalohydrolase MTHase EC 3.2.1.141 4-alpha-D- 1- 4 -alpha-D-glucano trehalose trehalohydrolase Maltooligosyl trehalose trehalohydrolase |
| Gene Name | treZ DR_0464 |
| Organism | Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Deinococcus-Thermus Deinococci Deinococcales Deinococcaceae Deinococcus Deinococcus radiodurans Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) |
| Enzyme Sequence | MTQTQPVTPTPPASFQTQHDPRTRLGATPLPGGAGTRFRLWTSTARTVAVRVNGTEHVMTSLGGGIYELELPVGPGARYLFVLDGVPTPDPYARFLPDGVHGEAEVVDFGTFDWTDADWHGIKLADCVFYEVHVGTFTPEGTYRAAAEKLPYLKELGVTAIQVMPLAAFDGQRGWGYDGAAFYAPYAPYGRPEDLMALVDAAHRLGLGVFLDVVYNHFGPSGNYLSSYAPSYFTDRFSSAWGMGLDYAEPHMRRYVTGNARMWLRDYHFDGLRLDATPYMTDDSETHILTELAQEIHELGGTHLLLAEDHRNLPDLVTVNHLDGIWTDDFHHETRVTLTGEQEGYYAGYRGGAEALAYTIRRGWRYEGQFWAVKGEEHERGHPSDALEAPNFVYCIQNHDQIGNRPLGERLHQSDGVTLHEYRGAAALLLPMTPLLFQGQEWAASTPFQFFSDHAGELGQAVSEGRKKEFGGFSGFSGEDVPDPQAEQTFLNSKLNWAEREGGEHARTLRLYRDLLRLRREDPVLHNRQRENLTTGHDGDVLWVRTVTGAGERVLLWNLGQDTRAVAEVKLPFTVPRRLLLHTEGREDLTLGAGEAVLVG |
| Enzyme Length | 600 |
| Uniprot Accession Number | Q9RX51 |
| Absorption | |
| Active Site | ACT_SITE 275; /note=Nucleophile; /evidence=ECO:0000305; ACT_SITE 308; /note=Proton donor; /evidence=ECO:0000305 |
| Activity Regulation | |
| Binding Site | BINDING 376; /note=Substrate; /evidence=ECO:0000305|PubMed:15784255 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan.; EC=3.2.1.141; |
| DNA Binding | |
| EC Number | 3.2.1.141 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan biosynthesis; trehalose biosynthesis. |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (33); Binding site (1); Chain (1); Compositional bias (1); Helix (30); Region (4); Site (1); Turn (2) |
| Keywords | 3D-structure;Carbohydrate metabolism;Cytoplasm;Glycosidase;Hydrolase;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (9) |
| Cross Reference PDB | 2BHU; 2BHY; 2BHZ; 2BXY; 2BXZ; 2BY0; 2BY1; 2BY2; 2BY3; |
| Mapped Pubmed ID | 16421442; |
| Motif | |
| Gene Encoded By | |
| Mass | 66,910 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.141; |