IED ID | IndEnz0001000384 |
Enzyme Type ID | amylase000384 |
Protein Name |
Holliday junction ATP-dependent DNA helicase RuvB EC 3.6.4.12 |
Gene Name | ruvB Cgl1660 cg1869 |
Organism | Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) |
Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Corynebacteriaceae Corynebacterium Corynebacterium glutamicum (Brevibacterium saccharolyticum) Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) |
Enzyme Sequence | MERTEFEIPGGIPPRRNGGQGRAADTNVDANLKPDEYDAEVTLRPKSLTEFIGQPKVRDQLSLVLTGAKNRGVVPDHVLLSGPPGLGKTTMAMIIAQELGTSLRMTSGPALERAGDLAAMLSNLMEGDVLFIDEIHRIARPAEEMLYMAMEDFRIDVIVGKGPGATSIPLEIPPFTLVGATTRSGMLTGPLRDRFGFTAQMEFYDVPDLTKVVKRTAKILDVGIDNDAAVEIASRSRGTPRIANRLLRRVRDFAEVHADGHITMGAANAALIVFDVDEVGLDRLDRAVLDALIRGHGGGPVGVNTLAVAVGEEPGTVEEVCEPYLVRAGMIARTGRGRVATAAAWRHLGLEPPEGTIGDY |
Enzyme Length | 360 |
Uniprot Accession Number | Q9AE09 |
Absorption | |
Active Site | |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; Evidence={ECO:0000255|HAMAP-Rule:MF_00016}; |
DNA Binding | |
EC Number | 3.6.4.12 |
Enzyme Function | FUNCTION: The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. {ECO:0000255|HAMAP-Rule:MF_00016}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | NP_BIND 82..89; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00016 |
Features | Chain (1); Erroneous initiation (2); Nucleotide binding (1); Region (1); Sequence conflict (3) |
Keywords | ATP-binding;DNA damage;DNA recombination;DNA repair;Helicase;Hydrolase;Nucleotide-binding;Reference proteome;SOS response |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 38,603 |
Kinetics | |
Metal Binding | |
Rhea ID | RHEA:13065 |
Cross Reference Brenda |