| IED ID | IndEnz0001000416 |
| Enzyme Type ID | amylase000416 |
| Protein Name |
Alpha-amylase EC 3.2.1.1 1,4-alpha-D-glucan glucanohydrolase |
| Gene Name | |
| Organism | Aeromonas hydrophila |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Aeromonadales Aeromonadaceae Aeromonas Aeromonas hydrophila |
| Enzyme Sequence | MKNTAGILAIAGMLIAPLAHADVILHAFNWKYSEVTAKADLIKGAGYKQVLISPPLKSSGNEWWARYQPQDLRLVDSPLGNKQDLEQLIAAMQARGIAVYADVVLNHMANESWKRNDLNYPGTELLGQYAANPDYYSRQRLFGDLGQNLLSASDFHPEGCITDWSDPGHVQYWRLCGGAGDKGLPDLDPNNWVVSQQQAYLKALKGMGIKGFRVDAVKHMSDYQINAVFTPEIKQGMHVFGEVITTGGAGSTDYERFLKPYLDNSGQGAYDFPLFASLRGALGYGGSMNQLADPGAYGQALPGNRAVTFAITHDIPTNDGFRYQILNQTDEKLAYAYLLGRDGGSPLVYSDHGETQDKDGLRWQDYYLRSDLKGMIRFHNAVQGQPMQLIGSGDCFVLFKRGKQGLVGVNKCDYEQEYWLDTAKFELNWYRNYKDVLDQSAVINVQSQWVRVAMPARRPPLAAE |
| Enzyme Length | 464 |
| Uniprot Accession Number | P22630 |
| Absorption | |
| Active Site | ACT_SITE 215; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 242; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | BINDING 107; /note=Substrate; /evidence=ECO:0000250; BINDING 213; /note=Substrate; /evidence=ECO:0000250; BINDING 247; /note=Substrate; via amide nitrogen; /evidence=ECO:0000250; BINDING 313; /note=Substrate; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.; EC=3.2.1.1; |
| DNA Binding | |
| EC Number | 3.2.1.1 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Region (1); Signal peptide (1); Site (1) |
| Keywords | Carbohydrate metabolism;Glycosidase;Hydrolase;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 51,656 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |