| IED ID | IndEnz0001000521 |
| Enzyme Type ID | amylase000521 |
| Protein Name |
G-type lectin S-receptor-like serine/threonine-protein kinase LECRK1 OsLecRK1 EC 2.7.11.1 OsRLCK134 |
| Gene Name | LECRK1 LECRK OsI_14840 |
| Organism | Oryza sativa subsp. indica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. indica (Rice) |
| Enzyme Sequence | MVALLLFPMLLQLLSPTCAQTQKNITLGSTLAPQGPASSWLSPSGDFAFGFRPVEGNTSFYLIAVWFNKISDKTVVWYAKNTDQDPSIVEVPSDSFLQLTNDGALSLKDRSGQEGWNPQVTGVAYASMRDTGNFVLLGADGTTKWQTFDMPSDTILPTQVIPCNKTRNKSLRARLDIDDYSSGRFLLDVQTDGNLALYLVAVPSGSKYQQYWSTDTTGNGSELVFSETGKVYFALTDGTQINISSDAGIGSMADYFHRATLDPDGVFRQYVYPKKANAGILGGETWTALSMQPQNICHAIVSDVGSGVCGFNSYCTFDGTRNQIASCQCPPWYKFFDEQKKYKGCKQDFQPHSCDLEEATALAQFELRPIYGVDWPLSDYEKYEPIGQDDCGRLCVIECFCAMAVYNQSTSTCWKKKLPLSNGNMADYVQRTVLLKVPSSNSSQFMISTSSNKWKRNRKHWVLGSSLILGTSILVNFALISIFLFGTYCRITTKKNIPLSQASSKSQLPLKTFTYKELEKATAGFHEILGAGASGVVYKGQLEDELKTNIAVKTIHKLQPETEKEFMVEVETIGQTFHKNLVRLLGFCNERAERLLVYEFMTNGPLNRLLFDNSRPHWNTRVHIALGVARGFLYLHDECSKQIIHCDIKPQNILLDDNLVAKISDFGLAKLLLTNQTRTKTGIRGTRGYVAPEWFKNIGISTKVDVYSFGVILLELVCCRRNVELEVVDEEQTIVTYWANDCYRSGRIDLLVEGDDEAIYDIKKVERFVTVALWCLQEDPSMRPNMLKVTQMLDGAVAIPSPPDPCSFISSLP |
| Enzyme Length | 813 |
| Uniprot Accession Number | A0A075F7E9 |
| Absorption | |
| Active Site | ACT_SITE 647; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU00159 |
| Activity Regulation | |
| Binding Site | BINDING 553; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00159 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1; Evidence={ECO:0000305}; CATALYTIC ACTIVITY: Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1; Evidence={ECO:0000305}; |
| DNA Binding | |
| EC Number | 2.7.11.1 |
| Enzyme Function | FUNCTION: Involved in innate immunity. Required for the expression of defense-related genes PR1A, LOX2 and CHS1 upon biotic stresses. Required for basal resistance to the fungal blast (M.grisea), bacterial blight (O.oryzae pv. oryzae, Xoo) and the herbivorous insect brown planthopper (N.lugens, BPH). May be involved in several defense signaling pathways. Involved in the promotion of seed germination. Required for the expression of alpha-amylase genes during seed germination (PubMed:15685292). Involved in resistance against the brown planthopper (BPH). Member of the BPH3 (BPH resistance locus 3) cluster which contains LECRK1, LECRK2 and LECRK3 (PubMed:25485617). {ECO:0000269|PubMed:15685292, ECO:0000269|PubMed:25485617}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 529..537; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00159 |
| Features | Active site (1); Binding site (1); Chain (1); Disulfide bond (5); Domain (4); Erroneous gene model prediction (1); Glycosylation (8); Nucleotide binding (1); Sequence caution (1); Sequence conflict (25); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | ATP-binding;Disulfide bond;EGF-like domain;Glycoprotein;Kinase;Lectin;Membrane;Nucleotide-binding;Plant defense;Receptor;Reference proteome;Serine/threonine-protein kinase;Signal;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | INDUCTION: Induced by infection with the fungal blast (M.grisea) and bacterial blight (X.oryzae pv. oryzae, Xoo). {ECO:0000269|PubMed:24033867}. |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass type I membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 90,770 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:17989; RHEA:46608 |
| Cross Reference Brenda |