| IED ID | IndEnz0001000534 |
| Enzyme Type ID | amylase000534 |
| Protein Name |
Trypsin inhibitor 2 MCoTI-II Trypsin inhibitor II |
| Gene Name | |
| Organism | Momordica cochinchinensis (Spiny bitter cucumber) (Muricia cochinchinensis) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Cucurbitales Cucurbitaceae Momordiceae Momordica Momordica cochinchinensis (Spiny bitter cucumber) (Muricia cochinchinensis) |
| Enzyme Sequence | SGSDGGVCPKILKKCRRDSDCPGACICRGNGYCG |
| Enzyme Length | 34 |
| Uniprot Accession Number | P82409 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Inhibits trypsin; probably participates in a plant defense mechanism. {ECO:0000305|PubMed:10801322}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (3); Cross-link (3); Disulfide bond (3); Helix (1); Peptide (1); Site (1) |
| Keywords | 3D-structure;Direct protein sequencing;Disulfide bond;Isopeptide bond;Knottin;Plant defense;Protease inhibitor;Secreted;Serine protease inhibitor |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: A cyclic succinimide probably forms by loss of water between Asp-4 and Gly-5, that can then rehydrate to either the original peptide bond or to a beta-aspartyl isopeptide bond. Three isoforms of MCoTI-II are detected, two with the parent molecular weight, corresponding to the unmodified and proposed isopeptide forms, and one with a molecular weight 18 Da lower, corresponding to a succinimide cross-linked form. {ECO:0000269|PubMed:10801322}.; PTM: This is a cyclic peptide. {ECO:0000269|PubMed:10801322}. |
| Signal Peptide | |
| Structure 3D | NMR spectroscopy (5); X-ray crystallography (2) |
| Cross Reference PDB | 1HA9; 1IB9; 2C4B; 2IT8; 2MT8; 2PO8; 4GUX; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 3,477 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |