| IED ID | IndEnz0001000547 |
| Enzyme Type ID | amylase000547 |
| Protein Name |
A-kinase anchor protein 5 AKAP-5 A-kinase anchor protein 150 kDa AKAP 150 P150 cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein |
| Gene Name | Akap5 Akap150 |
| Organism | Rattus norvegicus (Rat) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat) |
| Enzyme Sequence | METSVSEIQIETKDEKRPEAASPQKERQERKTATLCFKRRKKVNKKKAKAGSKTAEETEKHAPEAGGSGQRQPAGAWASIKRLVTHRKPSESAEKQKPSEAEMQPEDGALPKKKTKSKLKIPCIRFSRGAKRSRPSKLTEDSGYVRVQGEADDLEIKAQIQPDEQATQAKSTQGLQEDVIVRDGKEIQESHISNNVISGEHVIGIELELEKESSALRMRTPGSEKEAKVILVKQGVQVQEASVLENSAADSPQPVTSTAPLSPATTHQLGLEEPSDSIRESAPSGKDDGRRKTAAEEKKSGETALGQAEEASSVSQADKSVLSQAEEATVGHTEEATVIQAQSQAKEGKLSQAEEATVAQAKETVLSQAEEVKLSQIEEPAISQAKKATVGQAKEAYVSQAEEAIVGHTEKATMGQAEEATVGHIEKTTVGQAEEATVGQAEEATVGQAEEATVGQAEEATVGQAEEATVGQAGEATVSHIEKTTVGQAEEAIVGQAEEATVGQAEEATVGQAEEATVGQAEEATVDQAEEATVGQAEEATVGQAGEAAVGQAEEAIVAQAEEATVGQAGEATVGQAEKATVGQAEEPIVGQAEETVLRHASDLKVNGVDAEKPRSEESKRMEPIAIIITDTEISEFDVKKSKNVPKQFLISMENEQVGVFANDSDFEGRTSEQYETLLIETASSLVKNAIELSVEQLVNEMVSEDNQINTLFQ |
| Enzyme Length | 714 |
| Uniprot Accession Number | P24587 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Multivalent scaffold protein that anchors the cAMP-dependent protein kinase/PKA to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade. Plays a role in long term synaptic potentiation by regulating protein trafficking from the dendritic recycling endosomes to the plasma membrane and controlling both structural and functional plasticity at excitatory synapses. {ECO:0000250|UniProtKB:P24588}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (9); Lipidation (2); Modified residue (2); Motif (1); Region (5); Repeat (28); Sequence conflict (1) |
| Keywords | Calmodulin-binding;Cell junction;Endosome;Lipoprotein;Membrane;Palmitate;Phosphoprotein;Reference proteome;Repeat;Synapse |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Postsynaptic recycling endosome membrane {ECO:0000250|UniProtKB:P24588}; Lipid-anchor {ECO:0000250|UniProtKB:P24588}. Note=Associates with lipid rafts. {ECO:0000250|UniProtKB:P24588}. |
| Modified Residue | MOD_RES 4; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:D3YVF0; MOD_RES 22; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:22673903 |
| Post Translational Modification | PTM: Palmitoylated. Palmitoylation at Cys-36 and Cys-123 plays a key role in the targeting of AKAP5 to lipid rafts. Palmitoylation by ZDHHC2 is required for AKAP5 function in LTP-stimulated recycling endosome exocytosis. {ECO:0000250|UniProtKB:P24588}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10598575; 10939335; 12135903; 12150937; 12177200; 12542670; 12595241; 12754513; 12763907; 12826266; 12938160; 14732469; 15141163; 15458848; 15930126; 15951119; 16460836; 16510716; 16940053; 17279627; 17548344; 17640527; 18381233; 18701070; 19119138; 19169250; 19535604; 19767149; 19858198; 20395454; 20410303; 20694001; 21273417; 21569553; 21674494; 22343722; 22623657; 22789851; 22940692; 22976297; 23392692; 24121510; 25005497; 25451626; 25653285; 28331977; 29800717; 30102916; 33716104; |
| Motif | MOTIF 74..94; /note=AKAP CaM-binding; /evidence=ECO:0000255|PROSITE-ProRule:PRU01241 |
| Gene Encoded By | |
| Mass | 75,962 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |