| IED ID | IndEnz0001000549 |
| Enzyme Type ID | amylase000549 |
| Protein Name |
Acetoacetate decarboxylase AAD ADC EC 4.1.1.4 |
| Gene Name | adc CA_P0165 |
| Organism | Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Clostridia Eubacteriales Clostridiaceae Clostridium Clostridium acetobutylicum Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) |
| Enzyme Sequence | MLKDEVIKQISTPLTSPAFPRGPYKFHNREYFNIVYRTDMDALRKVVPEPLEIDEPLVRFEIMAMHDTSGLGCYTESGQAIPVSFNGVKGDYLHMMYLDNEPAIAVGRELSAYPKKLGYPKLFVDSDTLVGTLDYGKLRVATATMGYKHKALDANEAKDQICRPNYMLKIIPNYDGSPRICELINAKITDVTVHEAWTGPTRLQLFDHAMAPLNDLPVKEIVSSSHILADIILPRAEVIYDYLK |
| Enzyme Length | 244 |
| Uniprot Accession Number | P23670 |
| Absorption | |
| Active Site | ACT_SITE 115; /note=Schiff-base intermediate with acetoacetate; /evidence=ECO:0000269|PubMed:5946601 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=acetoacetate + H(+) = acetone + CO2; Xref=Rhea:RHEA:19729, ChEBI:CHEBI:13705, ChEBI:CHEBI:15347, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526; EC=4.1.1.4; |
| DNA Binding | |
| EC Number | 4.1.1.4 |
| Enzyme Function | FUNCTION: Catalyzes the conversion of acetoacetate to acetone and carbon dioxide. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.95.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (15); Chain (1); Helix (5); Mutagenesis (2); Sequence conflict (1); Turn (1) |
| Keywords | 3D-structure;Decarboxylase;Direct protein sequencing;Lyase;Plasmid;Reference proteome;Schiff base |
| Interact With | Itself |
| Induction | INDUCTION: By linear fatty acids (up to butyrate). |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 3BH2; |
| Mapped Pubmed ID | 19458715; |
| Motif | |
| Gene Encoded By | Plasmid pSOL1 |
| Mass | 27,537 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:19729 |
| Cross Reference Brenda | 4.1.1.4; |