| IED ID | IndEnz0001000552 |
| Enzyme Type ID | amylase000552 |
| Protein Name |
Alpha-amylase 2 EC 3.2.1.1 1,4-alpha-D-glucan glucanohydrolase |
| Gene Name | amyB DICTH_0636.1 |
| Organism | Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) |
| Taxonomic Lineage | cellular organisms Bacteria Dictyoglomi Dictyoglomia Dictyoglomales Dictyoglomaceae Dictyoglomus Dictyoglomus thermophilum Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) |
| Enzyme Sequence | MIYDDKIFGDLCHKEFLVEREVKKLEEIYLEEVLPEDPKPEDEIEFTFNCPLKFHITSGKIVKDNREIYTFNIQERKTQWNDSIFNFSEIIKIKIPPLKENGLYQIHLYEMNEKIYEQYLSIDNFEAPLWSEESIIYHIFIDRFAKDEKEVEYSENLKEKLGGNLKGILSRLDYIENLGINTIWISPIFKSTSYHGYDIEDYFEIDPIWGTKEDLKKLVREAFNRGIRIILDFVPNHMSYKNPIFQKALKDKNSNLRSWFIFKGEDYETFFGVKSMPKINLKNKEAIDYIINAAKYWIREFGISGYRMDHATGPDINFWSIFYYNLKSEFPETFYFGEIVETPKETKKYVGKFDGTLDFYLFKIIRDFFIGKRWSTKEFVKMIDLEEKFYGNKFKRISFLENHDSNRFLWVAKDKKLLRLASIFQFSINAIPIIYNGQEMGCSQYRDILEGNRTLHEHARLPIPWSDDKQDKELIDFYRQLVKIRKSHPALYKGTFIPIFSDMISFIKETQEESILVLINIEDKEEIFNLNGTYRDLFSGNIYTNSLKLGPMSAHLLLRIDH |
| Enzyme Length | 562 |
| Uniprot Accession Number | P14898 |
| Absorption | |
| Active Site | ACT_SITE 309; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 338; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.; EC=3.2.1.1; |
| DNA Binding | |
| EC Number | 3.2.1.1 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Metal binding (1); Sequence conflict (1); Site (1) |
| Keywords | Calcium;Carbohydrate metabolism;Cytoplasm;Direct protein sequencing;Glycosidase;Hydrolase;Metal-binding |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 67,027 |
| Kinetics | |
| Metal Binding | METAL 236; /note=Calcium; /evidence=ECO:0000255 |
| Rhea ID | |
| Cross Reference Brenda |