| IED ID | IndEnz0001000554 |
| Enzyme Type ID | amylase000554 |
| Protein Name |
Alpha-amylase isozyme 3E EC 3.2.1.1 1,4-alpha-D-glucan glucanohydrolase |
| Gene Name | AMY1.4 AMY3E Os08g0473600 LOC_Os08g36900 P0013B04.34-1 P0451G12.3-1 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MGKHHVTLCCVVFAVLCLASSLAQAQVLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSHSVSPQGYMPGRLYDLDASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGPDMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLGFDGWRLDFAKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGKPATAFDFTTKGILQAAVQGELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWPFPSDKVMQGYAYILTHPGIPCIFYDHVFDWNLQHEIATLAEIRSRNGIHAESTLDILKAEGDIYVAMIDGKVITKLGPRYDAGGIIPSDFHVVAHGNDYCVWEKEGLRVPAGRKHY |
| Enzyme Length | 437 |
| Uniprot Accession Number | P27934 |
| Absorption | |
| Active Site | ACT_SITE 204; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P00693; ACT_SITE 229; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P00693 |
| Activity Regulation | |
| Binding Site | BINDING 231; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 233; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P04063; BINDING 251; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 292; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 311; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 317; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 396; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693; BINDING 423; /note=Substrate; /evidence=ECO:0000250|UniProtKB:P00693 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.; EC=3.2.1.1; Evidence={ECO:0000250|UniProtKB:P00693}; |
| DNA Binding | |
| EC Number | 3.2.1.1 |
| Enzyme Function | FUNCTION: Important for breakdown of endosperm starch during germination. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (2); Binding site (8); Chain (1); Erroneous gene model prediction (2); Metal binding (13); Region (6); Signal peptide (1); Site (1) |
| Keywords | Alternative splicing;Calcium;Carbohydrate metabolism;Glycosidase;Hydrolase;Metal-binding;Reference proteome;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 22967050; 8021273; 8392395; |
| Motif | |
| Gene Encoded By | |
| Mass | 48,708 |
| Kinetics | |
| Metal Binding | METAL 116; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:P00693; METAL 133; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:P00693; METAL 136; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P00693; METAL 138; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P00693; METAL 142; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:P00693; METAL 152; /note=Calcium 3; /evidence=ECO:0000250|UniProtKB:P00693; METAL 163; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:P00693; METAL 166; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P00693; METAL 168; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P00693; METAL 171; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P00693; METAL 173; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:P00693; METAL 173; /note=Calcium 3; /evidence=ECO:0000250|UniProtKB:P00693; METAL 208; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P00693 |
| Rhea ID | |
| Cross Reference Brenda |