| IED ID | IndEnz0001000635 |
| Enzyme Type ID | amylase000635 |
| Protein Name |
1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic EC 2.4.1.18 Starch branching enzyme I Fragment |
| Gene Name | SBEII |
| Organism | Pisum sativum (Garden pea) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Fabales Fabaceae Papilionoideae 50 kb inversion clade NPAAA clade Hologalegina IRL clade Fabeae Pisum Pisum sativum (Garden pea) |
| Enzyme Sequence | ATTTTTTHNSKNKQYLAKQKPVELTLGYQNPNGCKVCSFGSKGSIYQKVSSGFKGVSVMTDDKSTMPSVEEDFENIGILNVDSSLEPFKDHFKYRLKRYLHQKKLIEEYEGGLQEFAKGYLKFGFNREEDGISYREWAPAAQEAQIIGDFNGWNGSNLHMEKDQFGVWSIQIPDADGNPAIPHNSRVKFRFKHSDGVWVDRIPAWIKYATVDPTRFAAPYDGVYWDPPLSERYQFKHPRPPKPKAPRIYEAHVGMSSSEPRINSYREFADDVLPRIRENNYNTVQLMAVMEHSYYASFWYHVTKPFFAVSSRSGSPEDLKYLIDKAHSLGLNVLMDVIHSHASNNVTDGLNGFDVGQSSQQSYFHAGDRGYHKLWDSRLFNYANWKSSFLLSNLRWWLEEYKFDGFRFDGVTSMLYHHHGINMAFTGDYNEYFSEETDVDAVVYLMLANSLVHDILPDATDIAEDVSGMPGLGRPVSEVGIGFDYRLAMAIPDKWIDYLKNKKDSEWSMKEISLNLTNRRYTEKCVSYAESHDQSIVGDKTIAFLLMDEEMYSSMSCLTMLSPTIERGISLHKMIHFITLALGGEGYLNFMGNEFGHPEWIDFPREGNGWSYEKCRLTQWNLVDTNHLRYKFMNAFDRAMNLLDDKFSILASTKQIVSSTNNEDKVIVFERGDLVFVFNFHPENTYEGYKVGCDLPGKYRVALDSDATEFGGHGRVGHDADQFTSPEGIPGIPETNFNNRPNSFKVLSPPHTCVVYYRVDERQEESNNPNLGSVEETFAAADTDVARIPDVSMESEDSNLDRIEDNSEDAVDAGILKVEREVVGDN |
| Enzyme Length | 826 |
| Uniprot Accession Number | Q41059 |
| Absorption | |
| Active Site | ACT_SITE 409; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 464; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.; EC=2.4.1.18; |
| DNA Binding | |
| EC Number | 2.4.1.18 |
| Enzyme Function | FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. May preferentially transfer long chains during branching. {ECO:0000269|PubMed:7894509}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan biosynthesis; starch biosynthesis. |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (1); Non-terminal residue (1); Region (1); Transit peptide (1) |
| Keywords | Amyloplast;Chloroplast;Direct protein sequencing;Plastid;Transferase;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast. Plastid, amyloplast {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 94,191 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |