Detail Information for IndEnz0001000638
IED ID IndEnz0001000638
Enzyme Type ID amylase000638
Protein Name 1,4-alpha-glucan branching enzyme GlgB 2
EC 2.4.1.18
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2
Alpha-
1-
4
-glucan branching enzyme 2
Glycogen branching enzyme 2
BE 2
Gene Name glgB2 SAV_7399
Organism Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces avermitilis Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Enzyme Sequence MMTHAGVLGTRFTVWAPNARGVRVCGNFCRWDGAAFPMRSLGSSGVWELFVPGIGEGELYKFEITRPDGTHTVRADPMARRAEVPPATASIVTESSYAWADGAWMAARGERPVHESPFSVYEVHLPSWRPGLTYRQLAEQLPAYVADLGFTHVELLPVAEHPFGGSWGYQVTGFYAPTARLGTPDDFKYLVDALHRAGVGVLMDWVPAHFPRDDWALAEFDGRPLYEPEDPQRAAHPDWGTLEFDYGRKEVRNFLVANAVYWCEEFHIDGLRVDAVASMLYLDYSREEGQWSPNEFGGRDNPDAVAFLQEMNATLYRRVPGVITIAEESTAWDGVTRATHDNGLGFGLKWNMGWMHDSLGYVQHEPVHRKYHHHEMTFSMVYAYSENYVLPISHDEVVHGKQALVSKMPGDWWQRRANHRAYLGFMWAHPGKQLLFMGQEFAQGAEWSPEHGPEWWLLDDEYHSAGDHRGMRDLVRDLNTLYRAEPALWERDTDPSGFAWVVGDAAEDNVFAFLRHAADGTPLLAVSNFSPVVRHDYRLGVPDDIPAWQEALNTDAARYGGSDLICPDPVKPESGEIRLTLPPLATVWLRPA
Enzyme Length 592
Uniprot Accession Number Q825Q8
Absorption
Active Site ACT_SITE 274; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_00685; ACT_SITE 327; /note=Proton donor; /evidence=ECO:0000255|HAMAP-Rule:MF_00685
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.; EC=2.4.1.18; Evidence={ECO:0000255|HAMAP-Rule:MF_00685};
DNA Binding
EC Number 2.4.1.18
Enzyme Function FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. {ECO:0000255|HAMAP-Rule:MF_00685}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan biosynthesis; glycogen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00685}.
nucleotide Binding
Features Active site (2); Chain (1)
Keywords Carbohydrate metabolism;Glycogen biosynthesis;Glycogen metabolism;Glycosyltransferase;Reference proteome;Transferase
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 66,806
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda