Detail Information for IndEnz0001000691
IED ID IndEnz0001000691
Enzyme Type ID amylase000691
Protein Name 1,4-alpha-glucan branching enzyme GlgB
EC 2.4.1.18
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-
1-
4
-glucan branching enzyme
Glycogen branching enzyme
BE
Gene Name glgB BcerKBAB4_4710
Organism Bacillus mycoides (strain KBAB4) (Bacillus weihenstephanensis)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus cereus group Bacillus mycoides Bacillus mycoides (strain KBAB4) (Bacillus weihenstephanensis)
Enzyme Sequence MDVINCEEVKRDEFHTEKYYDSYNIFGAHIVTEDGMRGVRCTVWAPHAKAMSVVGDFNEWDYEQHKMLQVTEEGIWSLFVPHIEENEIYKYAIETMDGDVILKADPYAVYAEVRPNTASVVFDIEGYEWNDKNWIRKRKKKSIYKEAMTVYELHFGSWKKKEDGALYSYREMAEELIPYVVEHQFTHIEIMPLVEHPYDRSWGYQGTGYYAATSRFGTPHDLMYFVDECHKYGIGVILDWVPGHFCKDAHGLYLFDGTPTYEYKDRDVQENLVWGTVNFDLGKREVRNFLISNALFWMKYFHIDGFRVDAVANMLYWNKEGKEQSNEHAVSFLRELNEAVFAEDEEFLMTAEDSTAWPLVTAPTYEGGLGFNYKWNMGWMNDVLKYMECAPEYRKYIHEKMTFSLIYAHSENFILPLSHDEVVHGKKSLLNKMPGDYWDKFAQLRLLYGYFFTHPGKKLLFMGGEFGQFDEWKDLEDLDWNLHDFEMHRNMHDYFKELIALYKRSKPLWQLDHSPEGFQWIDANNNEQSIFSFIRQGDKQEDALVVVCNFTKATYENYKVGVPDFEYYNEILNSDSAQYGGSGQVNKKRLKTILEPYHNQAAHVEITIPPFGVSILRPVKTRKGSKKQDGSKTKVRSNVTSRGKR
Enzyme Length 645
Uniprot Accession Number A9VMV8
Absorption
Active Site ACT_SITE 309; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_00685; ACT_SITE 352; /note=Proton donor; /evidence=ECO:0000255|HAMAP-Rule:MF_00685
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.; EC=2.4.1.18; Evidence={ECO:0000255|HAMAP-Rule:MF_00685};
DNA Binding
EC Number 2.4.1.18
Enzyme Function FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. {ECO:0000255|HAMAP-Rule:MF_00685}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan biosynthesis; glycogen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00685}.
nucleotide Binding
Features Active site (2); Chain (1); Region (1)
Keywords Carbohydrate metabolism;Glycogen biosynthesis;Glycogen metabolism;Glycosyltransferase;Transferase
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 75,819
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda