Detail Information for IndEnz0001000695
IED ID IndEnz0001000695
Enzyme Type ID amylase000695
Protein Name 1,4-alpha-glucan branching enzyme GlgB
EC 2.4.1.18
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-
1-
4
-glucan branching enzyme
Glycogen branching enzyme
BE
Gene Name glgB
Organism Bergeriella denitrificans (Neisseria denitrificans)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Betaproteobacteria Neisseriales Neisseriaceae Bergeriella Bergeriella denitrificans (Neisseria denitrificans)
Enzyme Sequence MNRNRHIRRGYHPEAGERQIIDSLFAATHSDPFAYLGRHRVNDEREAVRVLRPDAHHIDIIDRHTGAVIMPSEKIDERGLFAAVLPEHAPDYALLVTYHEGEAAVREEDDYRFGSALQHTDAWLLGEGTHLRPYETLGAHFAEMDGVSGVRFAVWAPNARRVSVIGEFNGWDSRRHAMRPHTGNGLWDIFIPGVGLNALYKFSVLDANGNIREKADPYAFGAELRPTTASVVRGLPAKAEAPAFRRRANSVEAPISIYEVHLGSWRRNPENNYWLTYTQLADELVNYVKDMGFTHIELLPLSEYPFDGSWGYQATGLYAPTSRFGSPDELKALIDAAHAAGISVILDWVAGHFPTDDHGLNTFDGTALYEHADPREGYHQDWNTLIYNFGRNEVKNFLQGNALYWIERFGFDGIRVDAVASMIYRNYSRKDGEWIPNRYGGSENLEAIAFLRQTNAVLKSETPGAGSFAEESTSFADVTREAGLNFDFKWNMGWMNDTLRYMQEDPVHRKYHHGKMTFGMMYQYSENFVLPLSHDEVVHGKRSLLGKMPGDCWQQFANLRAYYGFMYGFPGKKLLFMGNEFAQGREWNYQEGLDWHLLDEAGGWHKGVQDYVRDLNHIYTAHAPLYQLDQQPEGFEWLVADDSDNSVFVFERRDRAGNRIIVISNFTPVVREHYRFGVNAPGRYTEILNSDRTQYQGSGIANGADITAENVPSHGKAQSLSLTLPPLATVYLYQKAAPATEIQTALRADKQPAVKDKQAKAK
Enzyme Length 762
Uniprot Accession Number Q9RQI5
Absorption
Active Site ACT_SITE 417; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_00685; ACT_SITE 470; /note=Proton donor; /evidence=ECO:0000255|HAMAP-Rule:MF_00685
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.; EC=2.4.1.18; Evidence={ECO:0000255|HAMAP-Rule:MF_00685};
DNA Binding
EC Number 2.4.1.18
Enzyme Function FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. {ECO:0000255|HAMAP-Rule:MF_00685}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan biosynthesis; glycogen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00685}.
nucleotide Binding
Features Active site (2); Chain (1)
Keywords Carbohydrate metabolism;Glycogen biosynthesis;Glycogen metabolism;Glycosyltransferase;Transferase
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 86,367
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda 2.4.1.18;