Detail Information for IndEnz0001000723
IED ID IndEnz0001000723
Enzyme Type ID amylase000723
Protein Name Glycogen debranching enzyme
EC 3.2.1.196
Limit dextrin alpha-1,6-maltotetraose-hydrolase
Gene Name glgX YPO3941 y3887 YP_3303
Organism Yersinia pestis
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Yersiniaceae Yersinia Yersinia pseudotuberculosis complex Yersinia pestis
Enzyme Sequence MAVLTHGSPTPSGAHFDGKGINFTLFSAHAEQVTLCLFDEQGQERQIAMPARTGDIWHGYLPGGKPGQRYGYRVSGPFEPSRGHRFNPHKLLIDPRTRALEGKVGDDPRFTGGVSQPDVRDSAAALPKCLVIHEEYDWQGDKPPAIPWGNTVIYEAHVRGLTQLHPDIPPELRGTYAALAHPALIEHLKTLGITTLELLPVQFHIDEPRLQKMGLSNYWGYNVLAPFAVDPDYASGREGISPLRELRDAVKALHNAGIEVILDVVFNHSAELDVFGPTLCQRGIDNASYYWLTPDGEYDNITGCGNALRLSHPYVTQWVIDCLNYWRDSCHVDGFRFDLGTVLGRTPAFDQHAPLFAALAADERLSACKLIAEPWDIGLGGYQLGNFPTGFSEWNDQYRDAMRGFWLRGEVPRGTFAQHFAASSRLFEQRGRLPSASINQITAHDGFTLLDLLCFNQKHNQMNGEENRDGSDNNHSNNFGCEGLVADAAIWQRRKACQRALLTTLLLSQGTPMLLAGDEQGHSQQGNNNAYCQNNILTWLDWGSADRALMTFTADLIRLRQQIPALTQDQWWQSGDSNVQWLDSQGQALSDAAWEQGCQQQLQILLSQRWLVLINATDHECEMHLPEGEWEGIPPFGVSDHAERLTTWRGSAHTICVLIKRD
Enzyme Length 662
Uniprot Accession Number Q8ZA76
Absorption
Active Site ACT_SITE 338; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_01248; ACT_SITE 373; /note=Proton donor; /evidence=ECO:0000255|HAMAP-Rule:MF_01248
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages to branches with degrees of polymerization of three or four glucose residues in limit dextrin.; EC=3.2.1.196; Evidence={ECO:0000255|HAMAP-Rule:MF_01248};
DNA Binding
EC Number 3.2.1.196
Enzyme Function FUNCTION: Removes maltotriose and maltotetraose chains that are attached by 1,6-alpha-linkage to the limit dextrin main chain, generating a debranched limit dextrin. {ECO:0000255|HAMAP-Rule:MF_01248}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan degradation; glycogen degradation. {ECO:0000255|HAMAP-Rule:MF_01248}.
nucleotide Binding
Features Active site (2); Chain (1); Site (1)
Keywords Carbohydrate metabolism;Glycogen metabolism;Glycosidase;Hydrolase;Reference proteome
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 20711500;
Motif
Gene Encoded By
Mass 73,912
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda