Detail Information for IndEnz0001000805
IED ID IndEnz0001000805
Enzyme Type ID amylase000805
Protein Name 1,4-alpha-glucan branching enzyme GlgB
EC 2.4.1.18
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-
1-
4
-glucan branching enzyme
Glycogen branching enzyme
BE
Gene Name glgB
Organism Kitasatospora aureofaciens (Streptomyces aureofaciens)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Kitasatospora Kitasatospora aureofaciens (Streptomyces aureofaciens)
Enzyme Sequence MSAARQPSPTVRDKAAPEPAAPAAPKGARAPRARRAAPPHGVRPAPALAAEERARLLEGRHHDPHAVLGARTQRGGVAFRVLRPYAKAVTVVAKGLRTELVDEGDGLFSGLLPLTGVPDYRLLVTYDSDEIEVHDPYRFLPALGELDLHLIGEGRHEELWTALGSQPMEHQGVAGTRFTVWAPNALGVRVTGDFSYWDAVAYPMRSLGASGVWELFLPGVAEGALYKYEITRPDGGRTLRADPMARYAEVPPANASIVTASRYEWQDAEWMARRGALAPHQAPMSVYELHLASWRPGLSYRQLAEQLPAYVKELGFTHVELMPVAEHPFGGSWGYQVTGFYAPTSRMGTPDDFRFLVDALHRAGIGVIVDWVPAHFPRDDWALAEFDGRPLYEHQDPRRAAHPDWGTLEFDYGRKEVRNFLVANAVYWCQEFHVDGLRADAVASMLYLDYSRDEGDWSPNAHGGREDLDAVALLQEMNATVYRRFPGVVTIAEESTAWDGVTRPTDSGGLGFGLKWNMGWMHDTLRYVSKEPVHRKYHHHDMTFGMVYAFSENFVLPISHDEVVHGKRSLVSKMPGDWWQQRATHRAYLGFMWAHPGKQLLFMGQEFAQGSEWSETYGPDWWVLDSSYPAAGDHLGVRSLVRDLNRTYTASPALWERDSVPEGFAWVEADAADDNVFAFLRFARDGSPLLCVSNFSPVVRHGYRIGVPQEVGQWREVLNTDLEPYGGSGVHHARALRPEPVPAQGRAVSLRMTLPPMATVWLRP
Enzyme Length 764
Uniprot Accession Number P52980
Absorption
Active Site ACT_SITE 440; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 493; /note=Proton donor; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
DNA Binding
EC Number 2.4.1.18
Enzyme Function FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
nucleotide Binding
Features Active site (2); Chain (1); Region (1)
Keywords Carbohydrate metabolism;Glycogen biosynthesis;Glycogen metabolism;Glycosyltransferase;Transferase
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 85,325
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda