| IED ID | IndEnz0001000842 |
| Enzyme Type ID | amylase000842 |
| Protein Name |
1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic EC 2.4.1.18 Q-enzyme Starch-branching enzyme |
| Gene Name | SBE1 RBE1 Os06g0726400 LOC_Os06g51084 P0017G10.8-1 P0017G10.8-2 P0548E04.28-1 P0548E04.28-2 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MLCLTSSSSSAPAPLLPSLADRPSPGIAGGGGNVRLSVVSSPRRSWPGKVKTNFSVPATARKNKTMVTVVEEVDHLPIYDLDPKLEEFKDHFNYRIKRYLDQKCLIEKHEGGLEEFSKGYLKFGINTVDGATIYREWAPAAQEAQLIGEFNNWNGAKHKMEKDKFGIWSIKISHVNGKPAIPHNSKVKFRFRHGGGAWVDRIPAWIRYATFDASKFGAPYDGVHWDPPACERYVFKHPRPPKPDAPRIYEAHVGMSGEEPEVSTYREFADNVLPRIRANNYNTVQLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLVDKAHSLGLRVLMDVVHSHASNNVTDGLNGYDVGQNTHESYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNLRYWMDEFMFDGFRFDGVTSMLYHHHGINKGFTGNYKEYFSLDTDVDAIVYMMLANHLMHKLLPEATIVAEDVSGMPVLCRPVDEGGVGFDFRLAMAIPDRWIDYLKNKEDRKWSMSEIVQTLTNRRYTEKCIAYAESHDQSIVGDKTIAFLLMDKEMYTGMSDLQPASPTINRGIALQKMIHFITMALGGDGYLNFMGNEFGHPEWIDFPREGNNWSYDKCRRQWSLVDTDHLRYKYMNAFDQAMNALEEEFSFLSSSKQIVSDMNEKDKVIVFERGDLVFVFNFHPNKTYKGYKVGCDLPGKYRVALDSDALVFGGHGRVGHDVDHFTSPEGMPGVPETNFNNRPNSFKVLSPPRTCVAYYRVDEDREELRRGGAVASGKIVTEYIDVEATSGETISGGWKGSEKDDCGKKGMKFVFRSSDEDCK |
| Enzyme Length | 820 |
| Uniprot Accession Number | Q01401 |
| Absorption | |
| Active Site | ACT_SITE 409; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 464; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.; EC=2.4.1.18; |
| DNA Binding | |
| EC Number | 2.4.1.18 |
| Enzyme Function | FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan biosynthesis; starch biosynthesis. |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (1); Beta strand (28); Chain (1); Erroneous gene model prediction (1); Helix (35); Region (1); Sequence conflict (4); Transit peptide (1); Turn (6) |
| Keywords | 3D-structure;Alternative splicing;Amyloplast;Chloroplast;Direct protein sequencing;Glycosyltransferase;Plastid;Reference proteome;Starch biosynthesis;Transferase;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast. Plastid, amyloplast. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (3) |
| Cross Reference PDB | 3AMK; 3AML; 3VU2; |
| Mapped Pubmed ID | 11333304; 11598221; 12226172; 14526120; 16964521; 17147604; 17147623; 18254594; 18997409; 19593540; 19897896; 20305271; 21493662; 21494675; 22414443; 22720620; 22771800; 22870386; 23077797; 23846875; 24670252; 24908500; 25731081; 25979995; 26082161; 26860852; 8325505; 8360192; 9870704; |
| Motif | |
| Gene Encoded By | |
| Mass | 93,236 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 2.4.1.18; |