| IED ID | IndEnz0001000847 |
| Enzyme Type ID | amylase000847 |
| Protein Name |
Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase GMPMT EC 2.4.99.16 1- 4 -alpha-D-glucan:maltose-1-phosphate alpha-D-maltosyltransferase |
| Gene Name | glgE BPSL2074 |
| Organism | Burkholderia pseudomallei (strain K96243) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Betaproteobacteria Burkholderiales Burkholderiaceae Burkholderia pseudomallei group Burkholderia pseudomallei (Pseudomonas pseudomallei) Burkholderia pseudomallei (strain K96243) |
| Enzyme Sequence | METRPSFAPNLYFCDARLVGPLHAWPPLFERVAAWGFDHVLIGGLWAASRRGYPRHVADPDRPAESFATSLDATSALARLSDSAREHGLRIAVEVVVDRVAREHPLHDAHRAWYVVDERDDALIDPRTAALAHDVAHANVGSAAALDALADWWRARLGALADAGAAAFLVDAPQRMPAHWWAALLRALRQARADLPVIAGVPGREREALAQLACAGFDAAFSSLRWWDLRAPWFVEEHRLLRRVGAPIAFPDAFDGPRLAHDWRQAAPETIERAHRRALWTAAALGTGWLVPMGFERGVAVELMAREPRADAYRAALDSAPFDLSGAIAEANALRRATPALRGNGEIAQLTGADAPATVLLRGARTALEYDDEAALIAVNPDLAHPAAIAPCAALAGVPGGFTRFAPFADGRRPRMGALEPFALAAGACTLLRAQRARPVTTAPAEDRRGNRPGTRASVTAALAGERIAIERIEPVVDDGRFAVKRVIGERLAVRAAIFADGHARLAAAVQWRAADENGWHEARCAAEGNDAWRADIPLERLGRHLFRVIAWRDDWATLVDEIGKKHAAGQAVALELEEARRLAADVLTRAPEANPAALAVLREFAAALDAAPPDQRLALIGAPHVADAFAALRERAFATRDAPVFPVDVERRAARFASWYEMFPRSASDDVRRHGTFDDVVAHLPRIRDMGFDVLYFPPIHPIGTTARKGRNNSLQAAPDDVGSPYAIGSPAGGHTAVHPQLGSLDAFRRLVAAARAHDLEIALDFAVQCSPDHPWLTEHPGWFAWRPDGSLRYAENPPKRYQDIVNPDFYARDAMPALWIALRDVVLFWIDAGVRIFRVDNPHTKPLPFWAWMIADVRARHPDTVFLSEAFTRPSMMYRLAKLGFSQSYTYFTWRESKREFIDYLTELADGPAREYFRPNFFVNTPDINPRHLQQAPRTQFVIRAALAATLSGLWGMYSGFELCESDALPDSEEYRDAEKYELRARDWRRPGHIGDEIARLNRARRDNPALQTHLGIRFAHAPNDAVLVFSKATPAHDNVVVVAISLDPWHPQATDFTLDAALYRGWGIADGERLVAVDQTADHVETWHGRRHYVALDPHVRPFAIWRVAPAAGVARGARDDARDVPAQEVHER |
| Enzyme Length | 1136 |
| Uniprot Accession Number | Q63T93 |
| Absorption | |
| Active Site | ACT_SITE 842; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 871; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | BINDING 710; /note=Maltose 1-phosphate; /evidence=ECO:0000250; BINDING 770; /note=Maltose 1-phosphate; /evidence=ECO:0000250; BINDING 805; /note=Maltose 1-phosphate; /evidence=ECO:0000250; BINDING 843; /note=Maltose 1-phosphate; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-glucosyl](n) + alpha-maltose 1-phosphate = [(1->4)-alpha-D-glucosyl](n+2) + phosphate; Xref=Rhea:RHEA:42692, Rhea:RHEA-COMP:9584, Rhea:RHEA-COMP:10183, ChEBI:CHEBI:15444, ChEBI:CHEBI:43474, ChEBI:CHEBI:63576; EC=2.4.99.16; |
| DNA Binding | |
| EC Number | 2.4.99.16 |
| Enzyme Function | FUNCTION: Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)-glucans. Is involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (4); Chain (1); Erroneous initiation (1); Region (3); Site (1) |
| Keywords | Carbohydrate metabolism;Glycosyltransferase;Reference proteome;Transferase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 125,438 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:42692 |
| Cross Reference Brenda |