IED ID | IndEnz0001000872 |
Enzyme Type ID | amylase000872 |
Protein Name |
Glycogen debranching enzyme EC 3.2.1.196 Limit dextrin alpha-1,6-maltotetraose-hydrolase |
Gene Name | glgX ECIAI1_3577 |
Organism | Escherichia coli O8 (strain IAI1) |
Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli O8 (strain IAI1) |
Enzyme Sequence | MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQEHRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDGEFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKGLTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNYWGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPLFSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFRFDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEWNDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDCVCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVENRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPAGEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR |
Enzyme Length | 657 |
Uniprot Accession Number | B7M2J4 |
Absorption | |
Active Site | ACT_SITE 336; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_01248; ACT_SITE 371; /note=Proton donor; /evidence=ECO:0000255|HAMAP-Rule:MF_01248 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages to branches with degrees of polymerization of three or four glucose residues in limit dextrin.; EC=3.2.1.196; Evidence={ECO:0000255|HAMAP-Rule:MF_01248}; |
DNA Binding | |
EC Number | 3.2.1.196 |
Enzyme Function | FUNCTION: Removes maltotriose and maltotetraose chains that are attached by 1,6-alpha-linkage to the limit dextrin main chain, generating a debranched limit dextrin. {ECO:0000255|HAMAP-Rule:MF_01248}. |
Temperature Dependency | |
PH Dependency | |
Pathway | PATHWAY: Glycan degradation; glycogen degradation. {ECO:0000255|HAMAP-Rule:MF_01248}. |
nucleotide Binding | |
Features | Active site (2); Chain (1); Compositional bias (1); Region (1); Site (1) |
Keywords | Carbohydrate metabolism;Glycogen metabolism;Glycosidase;Hydrolase |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 73,599 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |