| IED ID | IndEnz0002000068 |
| Enzyme Type ID | protease000068 |
| Protein Name |
Probable ubiquitin carboxyl-terminal hydrolase MINDY-4 EC 3.4.19.12 Probable deubiquitinating enzyme MINDY-4 |
| Gene Name | Mindy4 Fam188b |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MDSLYVEEVAASLVREFLSRKGLNKTFVTMDQERPRCELSINSRNDLRKVLHLEFLYKENKAKEKPLRTNLELITRYFLDNVGNTDNSESQEVPIPAIPVPKKNNKLPLRHSETTLVNIYDLSDEDTGRRTSWSEAGKARHDSLDGDILGNFVSSKKPSHKSKAAHVDLGDSLPLVPAWEKVDQLHSSEPGIDVKKTMERTRPKSGLIVRGMMAGPVASSPQDSFRKRSLRRSSALSRKLQTPEEIQQQSEPFVHTPAYLGPQEVPDSSSDSVSRSPLGQLNELSIEKPNVTSSSQGLSQRDRPRLRSVSEDSPLGYSHTEGNSRMAQDQLERAFKRQGVQPPSLRKNQLVSDRTDDKPDALQLEDVEDELIKEDIVLFPPPSMLKLQTVSKPIDLSLAKEIKTLLFGSTFCCFSEEWKLQNFSFNDIASLKYGIVQNKGGPCGVLAAVQGCVLQKLLFEGDNRTNSNLRLQPSDAQRTRCLALAIADILWRAGGKEQAVVALASGTPHFSPTGKYKADGVLETLTLYSLTSSEDLVTFIQQSVHQFEAGPYGCILLTLSAILSRSLELVRQDFDVPTSHLIGAHGYCTQELVNLLLTGRAVSNVFNDVVELDSGDGNITLLRGIEARSDIGFLSLFEHYNVCQVGCFLKTPRFPIWVVCSESHFSILFSLQPELLCDWRSERLFDLYYYDGLANQQEEIRLTVDTTKTAPADSCSDLVPPLELCIRTKWKGASVNWNGSDPIL |
| Enzyme Length | 744 |
| Uniprot Accession Number | Q3UQI9 |
| Absorption | |
| Active Site | ACT_SITE 443; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q8N5J2; ACT_SITE 664; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:Q8N5J2 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q8NBR6}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Probable hydrolase that can remove 'Lys-48'-linked conjugated ubiquitin from proteins. {ECO:0000250|UniProtKB:Q8NBR6}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (1); Chain (1); Compositional bias (3); Modified residue (4); Region (1) |
| Keywords | Alternative splicing;Hydrolase;Phosphoprotein;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | MOD_RES 143; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:21183079; MOD_RES 220; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q4G0A6; MOD_RES 224; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q4G0A6; MOD_RES 295; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q4G0A6 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 17971504; 21267068; |
| Motif | |
| Gene Encoded By | |
| Mass | 82,937 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |