Detail Information for IndEnz0002000079
IED ID IndEnz0002000079
Enzyme Type ID protease000079
Protein Name Antitoxin phd
Addiction protein pdh
Prevent host death protein
Gene Name phd
Organism Escherichia phage P1 (Bacteriophage P1)
Taxonomic Lineage Viruses Duplodnaviria Heunggongvirae Uroviricota Caudoviricetes Caudovirales Myoviridae (phages with contractile tails) Punavirus Escherichia phage P1 (Bacteriophage P1)
Enzyme Sequence MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
Enzyme Length 73
Uniprot Accession Number Q06253
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system (PubMed:18398006, PubMed:24141193, PubMed:18757857). A labile antitoxin that binds to cognate doc toxin and neutralizes its ability to phosphorylate host EF-Tu. Does not reverse phosphorylation. Bacteriophage P1 lysogenizes bacteria as a low-copy number plasmid; phd and doc proteins function in unison to stabilize plasmid number by inducing a lethal response to P1 plasmid prophage loss (PubMed:8411153). {ECO:0000269|PubMed:18398006, ECO:0000269|PubMed:18757857, ECO:0000269|PubMed:24141193, ECO:0000269|PubMed:8411153}.; FUNCTION: Binds to its own promoter repressing its expression; toxin doc acts as a corepressor or derepressor depending on the ratio, repressing or inducing expression. {ECO:0000269|PubMed:20603017, ECO:0000269|PubMed:9829946}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Beta strand (4); Chain (1); Helix (5); Mutagenesis (3); Region (1); Turn (1)
Keywords 3D-structure;Reference proteome;Repressor;Toxin-antitoxin system;Transcription;Transcription regulation
Interact With Q06259
Induction
Subcellular Location
Modified Residue
Post Translational Modification PTM: Degraded by the ClpXP protease. {ECO:0000269|PubMed:7724551}.
Signal Peptide
Structure 3D X-ray crystallography (8)
Cross Reference PDB 3DD7; 3HRY; 3HS2; 3K33; 3KH2; 4ZLX; 4ZM0; 4ZM2;
Mapped Pubmed ID 27159580;
Motif
Gene Encoded By
Mass 8,133
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda