| IED ID | IndEnz0002000082 |
| Enzyme Type ID | protease000082 |
| Protein Name |
Plastoglobule-localized metallopeptidase 48, chloroplastic EC 3.4.24.- |
| Gene Name | PGM48 At3g27110 MOJ10.19 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAVSVSAPVLSLCYNQSGELSRSLGYRLPKKVGFSSGRRSVSYIGFGAEKVGRFRVRVPICRAVPPLLFKDLDADDFRHPFDKQNTLLLRAIPGLNEFGKALLGSMTEQIMLLENIGTSVLVSKNQLSDLHGLLVEAAEILNIEAPDLYVRQSPVPNAYTLAISGKKPFIVVHTSLIELLTSAELQAVLAHELGHLKCDHGVWLTFANILTLGAYTVPAFGQMIARTLEEQLLRWLRSAELTCDRAALLVAQDPKVVVSVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPLGWYIRNAQTSQLSHPLPVLRAREIDEWSRSLEYKSLLKRANRKSTVQKV |
| Enzyme Length | 344 |
| Uniprot Accession Number | Q9LSC4 |
| Absorption | |
| Active Site | ACT_SITE 192; /evidence=ECO:0000250|UniProtKB:O75844 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Metalloendopeptidase with a Zn-dependent proteolytic activity and substrate cleavage upstream of hydrophobic residues (PubMed:27895226). Positive regulator of senescence, probably by degrading CCD4, thus participating in the controlled removal of carotenoids from the thylakoid membrane during the senescence process (PubMed:27895226, PubMed:28534654). {ECO:0000269|PubMed:27895226, ECO:0000269|PubMed:28534654}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (3); Sequence conflict (1); Transit peptide (1); Transmembrane (4) |
| Keywords | Chloroplast;Hydrolase;Membrane;Metal-binding;Metalloprotease;Plastid;Protease;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast, plastoglobule {ECO:0000269|PubMed:27895226}. Plastid, chloroplast membrane {ECO:0000255}; Multi-pass membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 37,948 |
| Kinetics | |
| Metal Binding | METAL 191; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:O75844; METAL 195; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:O75844; METAL 240; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:O75844 |
| Rhea ID | |
| Cross Reference Brenda |