Detail Information for IndEnz0002000082
IED ID IndEnz0002000082
Enzyme Type ID protease000082
Protein Name Plastoglobule-localized metallopeptidase 48, chloroplastic
EC 3.4.24.-
Gene Name PGM48 At3g27110 MOJ10.19
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MAVSVSAPVLSLCYNQSGELSRSLGYRLPKKVGFSSGRRSVSYIGFGAEKVGRFRVRVPICRAVPPLLFKDLDADDFRHPFDKQNTLLLRAIPGLNEFGKALLGSMTEQIMLLENIGTSVLVSKNQLSDLHGLLVEAAEILNIEAPDLYVRQSPVPNAYTLAISGKKPFIVVHTSLIELLTSAELQAVLAHELGHLKCDHGVWLTFANILTLGAYTVPAFGQMIARTLEEQLLRWLRSAELTCDRAALLVAQDPKVVVSVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPLGWYIRNAQTSQLSHPLPVLRAREIDEWSRSLEYKSLLKRANRKSTVQKV
Enzyme Length 344
Uniprot Accession Number Q9LSC4
Absorption
Active Site ACT_SITE 192; /evidence=ECO:0000250|UniProtKB:O75844
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.24.-
Enzyme Function FUNCTION: Metalloendopeptidase with a Zn-dependent proteolytic activity and substrate cleavage upstream of hydrophobic residues (PubMed:27895226). Positive regulator of senescence, probably by degrading CCD4, thus participating in the controlled removal of carotenoids from the thylakoid membrane during the senescence process (PubMed:27895226, PubMed:28534654). {ECO:0000269|PubMed:27895226, ECO:0000269|PubMed:28534654}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Metal binding (3); Sequence conflict (1); Transit peptide (1); Transmembrane (4)
Keywords Chloroplast;Hydrolase;Membrane;Metal-binding;Metalloprotease;Plastid;Protease;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix;Zinc
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast, plastoglobule {ECO:0000269|PubMed:27895226}. Plastid, chloroplast membrane {ECO:0000255}; Multi-pass membrane protein {ECO:0000255}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 37,948
Kinetics
Metal Binding METAL 191; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:O75844; METAL 195; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:O75844; METAL 240; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:O75844
Rhea ID
Cross Reference Brenda