| IED ID | IndEnz0002000086 |
| Enzyme Type ID | protease000086 |
| Protein Name |
Pepsin B EC 3.4.23.2 |
| Gene Name | PGB PGNB |
| Organism | Canis lupus familiaris (Dog) (Canis familiaris) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Carnivora Caniformia Canidae (dog coyote wolf fox) Canis Canis lupus (Gray wolf) Canis lupus familiaris (Dog) (Canis familiaris) |
| Enzyme Sequence | MKIQVLVLVCLHLSEGVERIILKKGKSIRQVMEERGVLETFLRNHPKVDPAAKYLFNNDAVAYEPFTNYLDSYYFGEISIGTPPQNFLILFDTGSSNLWVPSTYCQSQACSNHNRFNPSRSSTYQSSEQTYTLAYGFGSLTVLLGYDTVTVQNIVIHNQLFGMSENEPNYPFYYSYFDGILGMAYSNLAVDNGPTVLQNMMQQGQLTQPIFSFYFSPQPTYEYGGELILGGVDTQFYSGEIVWAPVTREMYWQVAIDEFLIGNQATGLCSQGCQGIVDTGTFPLTVPQQYLDSFVKATGAQQDQSGNFVVNCNSIQSMPTITFVISGSPLPLPPSTYVLNNNGYCTLGIEVTYLPSPNGQPLWILGDVFLREYYTVFDMAANRVGFALSS |
| Enzyme Length | 390 |
| Uniprot Accession Number | Q8SQ41 |
| Absorption | |
| Active Site | ACT_SITE 92; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094; ACT_SITE 278; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Degradation of gelatin, little activity on hemoglobin. Specificity on B chain of insulin more restricted than that of pepsin A. Does not cleave 1-Phe-|-Val-2, 4-Gln-|-His-5 or 23-Gly-|-Phe-24.; EC=3.4.23.2; Evidence={ECO:0000269|PubMed:12147255}; |
| DNA Binding | |
| EC Number | 3.4.23.2 |
| Enzyme Function | FUNCTION: Hydrolyzes various peptides including beta-endorphin, insulin B chain, dynorphin A, and neurokinin A, with high specificity for the cleavage of the Phe-Xaa bonds. {ECO:0000269|PubMed:12147255}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (3); Domain (1); Propeptide (1); Signal peptide (1) |
| Keywords | Aspartyl protease;Digestion;Disulfide bond;Hydrolase;Protease;Reference proteome;Secreted;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 17128981; |
| Motif | |
| Gene Encoded By | |
| Mass | 43,497 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |