Detail Information for IndEnz0002000086
IED ID IndEnz0002000086
Enzyme Type ID protease000086
Protein Name Pepsin B
EC 3.4.23.2
Gene Name PGB PGNB
Organism Canis lupus familiaris (Dog) (Canis familiaris)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Carnivora Caniformia Canidae (dog coyote wolf fox) Canis Canis lupus (Gray wolf) Canis lupus familiaris (Dog) (Canis familiaris)
Enzyme Sequence MKIQVLVLVCLHLSEGVERIILKKGKSIRQVMEERGVLETFLRNHPKVDPAAKYLFNNDAVAYEPFTNYLDSYYFGEISIGTPPQNFLILFDTGSSNLWVPSTYCQSQACSNHNRFNPSRSSTYQSSEQTYTLAYGFGSLTVLLGYDTVTVQNIVIHNQLFGMSENEPNYPFYYSYFDGILGMAYSNLAVDNGPTVLQNMMQQGQLTQPIFSFYFSPQPTYEYGGELILGGVDTQFYSGEIVWAPVTREMYWQVAIDEFLIGNQATGLCSQGCQGIVDTGTFPLTVPQQYLDSFVKATGAQQDQSGNFVVNCNSIQSMPTITFVISGSPLPLPPSTYVLNNNGYCTLGIEVTYLPSPNGQPLWILGDVFLREYYTVFDMAANRVGFALSS
Enzyme Length 390
Uniprot Accession Number Q8SQ41
Absorption
Active Site ACT_SITE 92; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094; ACT_SITE 278; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Degradation of gelatin, little activity on hemoglobin. Specificity on B chain of insulin more restricted than that of pepsin A. Does not cleave 1-Phe-|-Val-2, 4-Gln-|-His-5 or 23-Gly-|-Phe-24.; EC=3.4.23.2; Evidence={ECO:0000269|PubMed:12147255};
DNA Binding
EC Number 3.4.23.2
Enzyme Function FUNCTION: Hydrolyzes various peptides including beta-endorphin, insulin B chain, dynorphin A, and neurokinin A, with high specificity for the cleavage of the Phe-Xaa bonds. {ECO:0000269|PubMed:12147255}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Disulfide bond (3); Domain (1); Propeptide (1); Signal peptide (1)
Keywords Aspartyl protease;Digestion;Disulfide bond;Hydrolase;Protease;Reference proteome;Secreted;Signal;Zymogen
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..16; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 17128981;
Motif
Gene Encoded By
Mass 43,497
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda