| IED ID | IndEnz0002000116 |
| Enzyme Type ID | protease000116 |
| Protein Name |
Pepsin B EC 3.4.23.2 |
| Gene Name | PGB |
| Organism | Monodelphis domestica (Gray short-tailed opossum) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Metatheria Didelphimorphia Didelphidae (opossums) Didelphinae Monodelphis (short-tailed opossums) Monodelphis domestica (Gray short-tailed opossum) |
| Enzyme Sequence | MKCLILALICLQLSEGLVVRQILHKGKSIRERMEENGVLEDFLRYNKKADPAAKFLFNKDAVAYEPITNYLDSFYFGEISIGTPPQNFLVLFDTGSSNLWVPSTYCQSQACSNHNRFSPSQSSTFTNGGQTYTLSYGSGSLTVVLGYDTVTVQNIVVSNQEFGLSESEPTSPFYYSDFDGILGMAYPAMAVGNSPTVMQGMLQQGQLSEPIFSFYFSRQPTHQYGGELILGGVDPQLYSGQITWTPVTQEVYWQIGIEEFAIGNQATGWCSQGCQAIVDTGTFLLAVPQQYMSAFLQATGAQQAQNGDFMVNCNYIQDMPTITFVINGSQFPLPPSAYVFNNNGYCRLGIEATYLPSPNGQPLWILGDVFLKEYYSVYDMANNRVGFAYSA |
| Enzyme Length | 391 |
| Uniprot Accession Number | Q689Z7 |
| Absorption | |
| Active Site | ACT_SITE 93; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094; ACT_SITE 279; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Degradation of gelatin, little activity on hemoglobin. Specificity on B chain of insulin more restricted than that of pepsin A. Does not cleave 1-Phe-|-Val-2, 4-Gln-|-His-5 or 23-Gly-|-Phe-24.; EC=3.4.23.2; Evidence={ECO:0000250|UniProtKB:Q8SQ41}; |
| DNA Binding | |
| EC Number | 3.4.23.2 |
| Enzyme Function | FUNCTION: Hydrolyzes various peptides including beta-endorphin, insulin B chain, dynorphin A, and neurokinin A, with high specificity for the cleavage of the Phe-Xaa bonds. {ECO:0000250|UniProtKB:Q8SQ41}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (3); Domain (1); Propeptide (1); Signal peptide (1) |
| Keywords | Aspartyl protease;Digestion;Disulfide bond;Hydrolase;Protease;Reference proteome;Secreted;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 43,281 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |