IED ID | IndEnz0002000116 |
Enzyme Type ID | protease000116 |
Protein Name |
Pepsin B EC 3.4.23.2 |
Gene Name | PGB |
Organism | Monodelphis domestica (Gray short-tailed opossum) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Metatheria Didelphimorphia Didelphidae (opossums) Didelphinae Monodelphis (short-tailed opossums) Monodelphis domestica (Gray short-tailed opossum) |
Enzyme Sequence | MKCLILALICLQLSEGLVVRQILHKGKSIRERMEENGVLEDFLRYNKKADPAAKFLFNKDAVAYEPITNYLDSFYFGEISIGTPPQNFLVLFDTGSSNLWVPSTYCQSQACSNHNRFSPSQSSTFTNGGQTYTLSYGSGSLTVVLGYDTVTVQNIVVSNQEFGLSESEPTSPFYYSDFDGILGMAYPAMAVGNSPTVMQGMLQQGQLSEPIFSFYFSRQPTHQYGGELILGGVDPQLYSGQITWTPVTQEVYWQIGIEEFAIGNQATGWCSQGCQAIVDTGTFLLAVPQQYMSAFLQATGAQQAQNGDFMVNCNYIQDMPTITFVINGSQFPLPPSAYVFNNNGYCRLGIEATYLPSPNGQPLWILGDVFLKEYYSVYDMANNRVGFAYSA |
Enzyme Length | 391 |
Uniprot Accession Number | Q689Z7 |
Absorption | |
Active Site | ACT_SITE 93; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094; ACT_SITE 279; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Degradation of gelatin, little activity on hemoglobin. Specificity on B chain of insulin more restricted than that of pepsin A. Does not cleave 1-Phe-|-Val-2, 4-Gln-|-His-5 or 23-Gly-|-Phe-24.; EC=3.4.23.2; Evidence={ECO:0000250|UniProtKB:Q8SQ41}; |
DNA Binding | |
EC Number | 3.4.23.2 |
Enzyme Function | FUNCTION: Hydrolyzes various peptides including beta-endorphin, insulin B chain, dynorphin A, and neurokinin A, with high specificity for the cleavage of the Phe-Xaa bonds. {ECO:0000250|UniProtKB:Q8SQ41}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Chain (1); Disulfide bond (3); Domain (1); Propeptide (1); Signal peptide (1) |
Keywords | Aspartyl protease;Digestion;Disulfide bond;Hydrolase;Protease;Reference proteome;Secreted;Signal;Zymogen |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 43,281 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |