Detail Information for IndEnz0002000132
IED ID IndEnz0002000132
Enzyme Type ID protease000132
Protein Name Puromycin-hydrolyzing enzyme
EC 3.-.-.-
Fragment
Gene Name
Organism Streptomyces morookaense (Streptoverticillium morookaense)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces morookaense (Streptoverticillium morookaense)
Enzyme Sequence VSTAPYGAWQSPID
Enzyme Length 14
Uniprot Accession Number P81801
Absorption
Active Site
Activity Regulation ACTIVITY REGULATION: Stimulated by DTT. Strongly inhibited by zinc ion, Fe(2+) ion, Cu(2+) ion, mercury ion, N-bromosuccinimide and N-ethylmaleimide. Partially inhibited by cobalt ion.
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.-.-.-
Enzyme Function FUNCTION: Inactivates puromycin by catalyzing the hydrolysis of the amide linkage between its aminonucleoside and O-methyl-L-tyrosine moieties. May have aminopeptidase activity. {ECO:0000269|Ref.2}.
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 45 degrees Celsius.;
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.0.;
Pathway
nucleotide Binding
Features Chain (1); Non-terminal residue (1)
Keywords Aminopeptidase;Direct protein sequencing;Hydrolase;Protease
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 1,492
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda