| IED ID | IndEnz0002000132 |
| Enzyme Type ID | protease000132 |
| Protein Name |
Puromycin-hydrolyzing enzyme EC 3.-.-.- Fragment |
| Gene Name | |
| Organism | Streptomyces morookaense (Streptoverticillium morookaense) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces morookaense (Streptoverticillium morookaense) |
| Enzyme Sequence | VSTAPYGAWQSPID |
| Enzyme Length | 14 |
| Uniprot Accession Number | P81801 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Stimulated by DTT. Strongly inhibited by zinc ion, Fe(2+) ion, Cu(2+) ion, mercury ion, N-bromosuccinimide and N-ethylmaleimide. Partially inhibited by cobalt ion. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.-.-.- |
| Enzyme Function | FUNCTION: Inactivates puromycin by catalyzing the hydrolysis of the amide linkage between its aminonucleoside and O-methyl-L-tyrosine moieties. May have aminopeptidase activity. {ECO:0000269|Ref.2}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 45 degrees Celsius.; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.0.; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-terminal residue (1) |
| Keywords | Aminopeptidase;Direct protein sequencing;Hydrolase;Protease |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 1,492 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |