| IED ID | IndEnz0002000135 |
| Enzyme Type ID | protease000135 |
| Protein Name |
Peptidase B EC 3.4.11.23 Aminopeptidase B |
| Gene Name | pepB STM2536 |
| Organism | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella Salmonella enterica (Salmonella choleraesuis) Salmonella enterica I Salmonella typhimurium Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
| Enzyme Sequence | MTEAMKITLSTQPADARWGDKATYSINNDGITLHLNGKDDLGLIQRAARKIDGLGIKQVALTGEGWDTERCWAFWAGYKGPKGVRTVMWPDLDDAQRQELDNRLTIIDWVRDTINAPAEELGPEQLAQRAVDLLCSVACDSVTYRITKGEDLREQNYMGLHTVGRGSERPPVLLALDYNPTGDKDAPVYACLVGKGITFDSGGYSIKQSAFMDSMKSDMGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIIRYRNGKNVEVMNTDAEGRLVLADGLIDASAQHPQLIIDMATLTGAAKTALGNDYHALFSFDDTLAGRLLTSAAQENEPFWRLPLAEFHRNQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYREGWLHIDCSATYRKAPVEQWAAGATGLGVRTIANLLTA |
| Enzyme Length | 427 |
| Uniprot Accession Number | Q9RF52 |
| Absorption | |
| Active Site | ACT_SITE 207; /evidence=ECO:0000255; ACT_SITE 281; /evidence=ECO:0000255 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa, from a peptide or arylamide. Xaa is preferably Glu or Asp but may be other amino acids, including Leu, Met, His, Cys and Gln.; EC=3.4.11.23; |
| DNA Binding | |
| EC Number | 3.4.11.23 |
| Enzyme Function | FUNCTION: Probably plays an important role in intracellular peptide degradation. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.5-9.5.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Metal binding (7); Mutagenesis (1) |
| Keywords | Aminopeptidase;Cytoplasm;Direct protein sequencing;Hydrolase;Manganese;Metal-binding;Protease;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 46,355 |
| Kinetics | |
| Metal Binding | METAL 195; /note=Manganese 2; /evidence=ECO:0000250; METAL 200; /note=Manganese 1; /evidence=ECO:0000250; METAL 200; /note=Manganese 2; /evidence=ECO:0000250; METAL 218; /note=Manganese 2; /evidence=ECO:0000250; METAL 277; /note=Manganese 1; /evidence=ECO:0000250; METAL 279; /note=Manganese 1; /evidence=ECO:0000250; METAL 279; /note=Manganese 2; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.11.1; |