Detail Information for IndEnz0002000147
IED ID IndEnz0002000147
Enzyme Type ID protease000147
Protein Name Peptidase B
EC 3.4.11.23
Aminopeptidase B
Gene Name pepB VC_0755
Organism Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Vibrio cholerae Vibrio cholerae O1 Vibrio cholerae O1 biovar El Tor Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Enzyme Sequence MSTQMSVFLSTQAAQPQWGEKALISFAEQGATIHLQQTQDFSAIQRAARKLDNQGIRTAFLAGEGWDLESIWAFYQGYRDAKKRNTVEWKALAAAEQVELEARIKATDWTRDIINKSAEEVAPRQLATMAAEFIKSLAPDHVSYRIVKDKDLLTEGWEGIYAVGRGSERTSAMLQLDYNPTGDENAPVFACLVGKGITFDSGGYSLKPSNMMSAMKADMGGSGMITGALGLAIMRGFNKRVKLILCCAENMVSGRALKLGDIITYKNGKTVEIMNTDAEGRLVLADGLIYASEQKPQLIIDCATLTGAAKNALGNDYHALLSYDESLSQQALAAAKEENEALWALPLAEFHREMLPSNFADLSNISNGDYTPGASTAAAFLSYFVEGYQKGWLHFDCSATYRKSASDKWAAGATGMGVKMLARILMQQA
Enzyme Length 429
Uniprot Accession Number Q9KTX5
Absorption
Active Site ACT_SITE 207; /evidence=ECO:0000255|HAMAP-Rule:MF_00504; ACT_SITE 281; /evidence=ECO:0000255|HAMAP-Rule:MF_00504
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa, from a peptide or arylamide. Xaa is preferably Glu or Asp but may be other amino acids, including Leu, Met, His, Cys and Gln.; EC=3.4.11.23; Evidence={ECO:0000255|HAMAP-Rule:MF_00504};
DNA Binding
EC Number 3.4.11.23
Enzyme Function FUNCTION: Probably plays an important role in intracellular peptide degradation. {ECO:0000255|HAMAP-Rule:MF_00504}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Erroneous initiation (1); Metal binding (7)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00504}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 46,759
Kinetics
Metal Binding METAL 195; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00504; METAL 200; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00504; METAL 200; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00504; METAL 218; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00504; METAL 277; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00504; METAL 279; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00504; METAL 279; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00504
Rhea ID
Cross Reference Brenda